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90A1 Sigma

α-Naphthyl Acetate (Non-Specific Esterase)

Select reagents packaged in gelatin capsules.

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Properties

Related Categories Cell Biology, Cytochemistry Products, Hematology and Histology More...
Quality Level   GMP-IVD
shelf life   Expiry date on the label.
IVD   for in vitro diagnostic use
shipped in   wet ice
storage temp.   2-8°C

Description

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.

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Tissue Diagnostics

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Biomedical Applications

Kit component also available separately

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Safety & Documentation

Safety Information

Signal word 
Danger
Precautionary statements 
RIDADR 
UN 3316 9
Flash Point(F) 
104 °F
Flash Point(C) 
40 °C
Protocols & Articles
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