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90C2 Sigma

Naphthol AS-D Chloroacetate (Specific Esterase) Kit

Synonym: Leder Stain

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Properties

Related Categories Cell Biology, Cytochemistry Products, Hematology and Histology More...
Quality Level   GMP-IVD
description   Select reagents packaged in gelatin capsules
shelf life   Expiry date on the label.
IVD   for in vitro diagnostic use
shipped in   wet ice
storage temp.   2-8°C

Description

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. With the 90C2 kit, Naphthol AS-D Chloroacetate is enzymatically hydrolyzed by "specific esterase, " liberating a free naphthol compound. This then couples with a diazonium salt, forming highly colored deposits at sites of enzyme activity. This enzyme is usually considered specific for cells of granulocytic lineage. May be used to detect neutrophils in peripheral blood, bone marrow or tissue sections which have been paraffin embedded.

Price and Availability


All labs need water

Kit component also available separately

Description

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Acid hematoxylin solution 100 mL SDS 2852
Citrate Concentrate 20 mL SDS 3861
Naphthol AS-D chloroacetate 10 x 20 mg SDS 905
Trizmal buffer, pH 6.3(concentrate), 0.2 M 50 mL SDS 903C
Fast Corinth V Salt (9015) 50 mL SDS    

Kit component only

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Dimethyl formamide (9010) 25 mL SDS    
Fast Corinth V Salt (9015) 10 capsules SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Precautionary statements 
RIDADR 
UN 3316 9
Protocols & Articles
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