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91051509 Sigma

2D12

Reactivity: Anti-Yellow Fever Virus

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   P3X63Ag8.653
Reactivity   Anti-Yellow Fever Virus
application(s)   cell culture | mammalian: suitable
isotype   IgG2a, kappa
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Yellow fever virus, strain 17D

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Mouse P3X63Ag8.653 x Mouse BALB/c spleen

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-mercaptoethanol (2ME) + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

The antibody is directed against the envelope protein of the wild type and vaccine strains of yellow fever virus. It does not cross react with other flaviviruses.

Price and Availability


Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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M7145 MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
R0883 RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M6250 2-Mercaptoethanol, ≥99.0%
S8636 Sodium pyruvate solution, 100 mM, sterile-filtered, BioReagent, suitable for cell culture

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