91062702 Sigma

RAW 264.7 Cell Line murine

Macrophage from blood

Properties

Related Categories Cell Culture, ECACC Cell Lines, O-R More...
biological source   Blood from mouse
growth mode   Semi-adherent
karyotype   Not specified
morphology   Macrophage
products   Lysozyme
receptors   Immunoglobulin, complement
shipped in   dry ice
storage temp.   −196°C

Description

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Application

Metabolic studies

Culture Medium

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

Cell Line Description

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

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