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91062702 Sigma

RAW 264.7 Cell Line murine

Macrophage from blood

Properties

Related Categories Cell Culture, ECACC Cell Lines, O-R More...
biological source   Blood from mouse
growth mode   Semi-adherent
karyotype   Not specified
morphology   Macrophage
products   Lysozyme
receptors   Immunoglobulin, complement
application(s)   cell culture: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Frequently Asked Questions

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Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Metabolic studies

Cell Line Origin

Mouse monocyte macrophage

Culture Medium

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

Cell Line Description

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

Price and Availability


Transfection Reagents Free Sample!

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Required for Optimal Growth

With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Fetal Bovine Serum

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

<SC>L</SC>-Glutamine solution

200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

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