|Related Categories||Cell Culture, ECACC Cell Lines, O-R More...|
|biological source||Blood from mouse|
|shipped in||dry ice|
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Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.
Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.
Certificate of Analysis
For many decades cell lines have been fundamentally important tools in many fields of biomedical research, including cancer research. Over previous decades many billions of dollars have been spent, a...
Edward Burnett and Liz Penn, European Collection of Cell Culture (ECACC®)
Don Finley, Market Segment Manager, Sigma® Life Science
Biofiles, Vol. 8, No. 16
Keywords: Amplification, Cancer, Cell culture, Polymerase chain reaction
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Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without
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