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91072520 Sigma

IL-A30

Reactivity: Anti-IgM (monomorphic epitope), bovine

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   P3X63Ag8.653
Reactivity   Anti-IgM (monomorphic epitope), bovine
application(s)   cell culture | mammalian: suitable
isotype   IgG1
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Bovine PBMs, depleted of T lymphocytes

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Evaluation of bovine immunoglobulin levels and lymphocyte populations

Cell Line Origin

Mouse P3X63Ag8.653 x Mouse BALB/c spleen

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells may require mouse macrophage feeder cells upon resuscitation.

Cell Line Description

The antibody is directed against bovine IgM. It detects serum IgM and also IgM+ B cells. It does not react with bovine IgG.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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recommended

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Description

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R0883 RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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