91091004 Sigma

SK-OV-3 Cell Line human

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   Ovary from human
description   Human Caucasian ovary adenocarcinoma
growth mode   Adherent
karyotype   Hypodiploid to hypotetraploid
morphology   Epithelial
shipped in   dry ice
storage temp.   −196°C

Description

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 12
D5S818: 11
D7S820: 13,14
THO1: 9,9.3
TPOX: 8,11
vWA: 17,18

Culture Medium

McCoy′s 5a + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-6x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells.

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