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91228 Sigma

Trichloroacetic acid

BioUltra, ≥99.5% (T)

Synonym: TCA

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Properties

Related Categories Acids and Bases, Alphabetical, Biochemicals and Reagents, Denaturation
vapor density   <1 (vs air)
vapor pressure   1 mmHg ( 51 °C)
InChI Key   YNJBWRMUSHSURL-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (T)
impurities   insoluble matter, passes filter test
ign. residue   ≤0.05%
refractive index   n20/D 1.62(lit.)
pH   <1.0 (25 °C, 0.5 M in H2O)
bp   196 °C(lit.)
mp   54-58 °C(lit.)
solubility   H2O: 0.5 M at 20 °C clear, colorless
density   1.62 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤30 mg/kg
  nitrate (NO3-): ≤20 mg/kg
  phosphate (PO43-): ≤10 mg/kg
  sulfate (SO42-): ≤100 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   0.5 M in H2O
UV absorption   λ: 260 nm Amax: 1.60
  λ: 280 nm Amax: 0.15

Description

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Other Notes

Concentration of protein by quantitative precipitation; Cytochemical demonstration of DNA with a thiazine dye

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 1839 8 / PGII
WGK Germany 
2
RTECS 
AJ7875000
Flash Point(F) 
>235.4 °F
Flash Point(C) 
>113 °C
Protocols & Articles

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Peer-Reviewed Papers
15

References

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