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91A Sigma

α-Naphthyl Acetate (Non-Specific Esterase)

Kit formulated with all liquid reagents.

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Properties

Related Categories Cell Biology, Cytochemistry Products, Hematology and Histology More...
Quality Level   GMP-IVD
shelf life   Expiry date on the label.
IVD   for in vitro diagnostic use
shipped in   wet ice
storage temp.   2-8°C

Description

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.

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Tissue Diagnostics

All labs need water

Biomedical Applications

Kit component also available separately

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α-Naphthyl acetate solution 10 mL SDS 916
Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM 50 mL SDS 915
Fast Blue BB Base solution 10 mL SDS 917
Sodium nitrite solution 10 mL SDS 914

Kit component only

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Hematoxylin solution, Gill No. 3 50 mL SDS    
TRIZMAL™ 7.6 Concentrate 50 mL SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 3316 9
WGK Germany 
3
Flash Point(F) 
96.8 °F
Flash Point(C) 
36 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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90A1 α-Naphthyl Acetate (Non-Specific Esterase), Select reagents packaged in gelatin capsules.

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Description

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915 Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM
91C Naphthol AS-D Chloroacetate (Specific Esterase) Kit
916 α-Naphthyl acetate solution
08168 Timestrip Plus 8 °C
917 Fast Blue BB Base solution
914 Sodium nitrite solution

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