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91C Sigma

Naphthol AS-D Chloroacetate (Specific Esterase) Kit

Synonym: Leder Stain

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Properties

Related Categories Cell Biology, Cytochemistry Products, Hematology and Histology More...
Quality Level   GMP-IVD
description   Kit formulated with all liquid reagents
shelf life   Expiry date on the label.
IVD   for in vitro diagnostic use
shipped in   wet ice
storage temp.   2-8°C

Description

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. With the 91C kit, Naphthol AS-D Chloroacetate is enzymatically hydrolyzed by "specific esterase, " liberating a free naphthol compound. This then couples with a diazonium compound, forming highly colored deposits at sites of enzyme activity. This enzyme is usually considered specific for cells of granulocytic lineage. May be used to detect neutrophils in peripheral blood, bone marrow or tissue sections which have been paraffin embedded.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications

Kit component also available separately

Description

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Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM 50 mL SDS 915
Fast Red Violet LB Base solution 10 mL SDS 912
Naphthol AS-D Chloroacetate solution 50 mL SDS 911
Naphthol AS-D Chloroacetate solution 50 mL SDS 911
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
Supplemental Hazard Statements 
May form explosive peroxides.
RIDADR 
UN 3316 9
Protocols & Articles
Peer-Reviewed Papers
15

References

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