Properties
| Related Categories | Cell Culture, ECACC Cell Lines, J-N More... |
| biological source | Breast from human |
| growth mode | Adherent |
| karyotype | Modal no.′s 62 and 64, near triploid |
| morphology | Epithelial |
| shipped in | dry ice |
| storage temp. | −196°C |
Description
Frequently Asked Questions
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Application
MDA-MB-231 was isolated from pleural effusions of a Caucasian breast cancer patient.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18
Culture Medium
L15+ 2mM Glutamine + 15% Foetal Bovine SerumFBS / FCS.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 37°C. CO2 is not required.
Cell Line Description
Isolated from pleural effusions of a breast cancer patient.
Price and Availability
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