Synonym: Manganese-dependent lignin peroxidase, MnP, Peroxidase, manganese
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This product is supplied as a mixture of isozymes, mol. mass (40-65 kDa).
One unit corresponds to the amount of enzyme, which oxidizes 1 μmole Mn2+ per minute to Mn3+ at pH 4.5 and 25 °C
only partially soluble in water or buffer
Manganese peroxidase from white-rot fungus (Phanerochaete chrysosporium) is from the peroxidase family and is used to oxidize manganese. It may be used to study wound healing1.
Manganese peroxidase catalyzes the oxidation of Mn2+ to Mn3+. Mn3+ is then in complex with oxalate, which is able to oxidize lignin. Manganese peroxidase from Phanerochaete chrysosporium binds 2 calcium ions per subunit. It binds 1 heme B (iron-protoporphyrin IX) group per subunit 1.
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Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
Detoxification of azo dyes mediated by cell-free supernatant culture with manganese-dependent peroxidase activity: effect of Mn2+ concentration and H2O2 dose. Contreras E, Urra J, Vásquez C, et al. Biotechnol. Prog. 28(1), 114-20, (2012)
Comparative evaluation of manganese peroxidase- and Mn(III)-initiated peroxidation of C18 unsaturated fatty acids by different methods. Kapich AN, Korneichik TV, Hammel KE, et al. Enzyme Microb. Technol. 49(1), 25-9, (2011)
The synergistic effect on production of lignin-modifying enzymes through submerged co-cultivation of Phlebia radiata, Dichomitus squalens and Ceriporiopsis subvermispora using agricultural residues. Dong YC, Wang W, Hu ZC, et al. Bioprocess Biosyst. Eng. 35(5), 751-60, (2012)
Heterologous expression of manganese peroxidase in Aspergillus niger and its effect on phenanthrene removal from soil. Cortés-Espinosa DV, Absalón ÁE, Sanchez N, et al. J. Mol. Microbiol. Biotechnol. 21(3-4), 120-9, (2011)
Continuous treatment of coloured industry wastewater using immobilized Phanerochaete chrysosporium in a rotating biological contactor reactor. Pakshirajan K and Kheria S J. Environ. Manage. 101, 118-23, (2012)
Optimization of culture medium composition for manganese peroxidase and tyrosinase production during Reactive Black 5 decolourization by the yeast Trichosporon akiyoshidainum. Martorell MM, Pajot HF, Rovati JI, et al. Yeast 29(3-4), 137-44, (2012)
Improvement of ligninolytic properties in the hyper lignin-degrading fungus Phanerochaete sordida YK-624 using a novel gene promoter. Sugiura T, Mori T, Kamei I, et al. FEMS Microbiol. Lett. 331(1), 81-8, (2012)
Kinetic and thermodynamic characterization of the functional properties of a hybrid versatile peroxidase using isothermal titration calorimetry: Insight into manganese peroxidase activation and lignin peroxidase inhibition. Ertan H, Siddiqui KS, Muenchhoff J, et al. Biochimie 94(5), 1221-31, (2012)
Predominance of a versatile-peroxidase-encoding gene, mnp4, as demonstrated by gene replacement via a gene targeting system for Pleurotus ostreatus. Salame TM, Knop D, Tal D, et al. Appl. Environ. Microbiol. 78(15), 5341-52, (2012)
Alkadienyl and alkenyl itaconic acids (ceriporic acids G and H) from the selective white-rot fungus Ceriporiopsis subvermispora: a new class of metabolites initiating ligninolytic lipid peroxidation. Nishimura H, Sasaki M, Seike H, et al. Org. Biomol. Chem. 10(31), 6432-42, (2012)
Kinetic properties of manganese peroxidase from the mushroom Stereum ostrea and its ability to decolorize dyes. Praveen K, Usha KY, Viswanath B, et al. J. Microbiol. Biotechnol. 22(11), 1540-8, (2012)
Effect of copper, nutrient nitrogen, and wood-supplement on the production of lignin-modifying enzymes by the white-rot fungus Phlebia radiata. Mäkelä MR, Lundell T, Hatakka A, et al. Fungal Biol. 117(1), 62-70, (2013)
Manganese peroxidase from the basidiomycete Phanerochaete chrysosporium: spectral characterization of the oxidized states and the catalytic cycle. Wariishi H, Akileswaran L, and Gold MH Biochemistry 27(14), 5365-70, (1988)
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