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93014 Sigma

Manganese peroxidase from white-rot fungus (Phanerochaete chrysosporium)

powder, light brown, ≥20 U/g

Synonym: Manganese-dependent lignin peroxidase, MnP, Peroxidase, manganese

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Description

Other Notes

This product is supplied as a mixture of isozymes, mol. mass (40-65 kDa).

Unit Definition

One unit corresponds to the amount of enzyme, which oxidizes 1 μmole Mn2+ per minute to Mn3+ at pH 4.5 and 25 °C

Physical form

only partially soluble in water or buffer

Application

Manganese peroxidase from white-rot fungus (Phanerochaete chrysosporium) is from the peroxidase family and is used to oxidize manganese. It may be used to study wound healing1.

Biochem/physiol Actions

Manganese peroxidase catalyzes the oxidation of Mn2+ to Mn3+. Mn3+ is then in complex with oxalate, which is able to oxidize lignin. Manganese peroxidase from Phanerochaete chrysosporium binds 2 calcium ions per subunit. It binds 1 heme B (iron-protoporphyrin IX) group per subunit 1.

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Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

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Peer-Reviewed Papers

References

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1. Reorganizing the protein space at the Universal Protein Resource (UniProt) The UniProt Consortium Nucleic Acids Res. 40, D71-D75, (2012)

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Fungal laccase, manganese peroxidase and lignin peroxidase: gene expression and regulation. Janusz G, Kucharzyk KH, Pawlik A, et al. Enzyme Microb. Technol. 52(1), 1-12, (2013)

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Predominance of a versatile-peroxidase-encoding gene, mnp4, as demonstrated by gene replacement via a gene targeting system for Pleurotus ostreatus. Salame TM, Knop D, Tal D, et al. Appl. Environ. Microbiol. 78(15), 5341-52, (2012)

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Lignin-degrading enzyme activities. Chen YR, Sarkanen S, and Wang YY Methods Mol. Biol. 908, 251-68, (2012)

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[Isolation and purification of Mn-peroxidase from Azospirillum brasilense Sp245]. Kupriashina MA, Selivanov NIu, and Nikitina VE Prikl. Biokhim. Mikrobiol. 48(1), 23-6, (2012)

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Comparison of ligninase-I and peroxidase-M2 from the white-rot fungus Phanerochaete chrysosporium. Paszczyński A, Huynh VB, and Crawford R Arch. Biochem. Biophys. 244(2), 750-65, (1986)

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Continuous treatment of coloured industry wastewater using immobilized Phanerochaete chrysosporium in a rotating biological contactor reactor. Pakshirajan K and Kheria S J. Environ. Manage. 101, 118-23, (2012)

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Alkadienyl and alkenyl itaconic acids (ceriporic acids G and H) from the selective white-rot fungus Ceriporiopsis subvermispora: a new class of metabolites initiating ligninolytic lipid peroxidation. Nishimura H, Sasaki M, Seike H, et al. Org. Biomol. Chem. 10(31), 6432-42, (2012)

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Comparative evaluation of manganese peroxidase- and Mn(III)-initiated peroxidation of C18 unsaturated fatty acids by different methods. Kapich AN, Korneichik TV, Hammel KE, et al. Enzyme Microb. Technol. 49(1), 25-9, (2011)

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Oil-in-water microemulsions enhance the biodegradation of DDT by Phanerochaete chrysosporium. Zheng G, Selvam A, and Wong JW Bioresour. Technol. 126, 397-403, (2012)

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Kraft lignin biodegradation by Novosphingobium sp. B-7 and analysis of the degradation process. Chen Y, Chai L, Tang C, et al. Bioresour. Technol. 123, 682-5, (2012)

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Effect of copper, nutrient nitrogen, and wood-supplement on the production of lignin-modifying enzymes by the white-rot fungus Phlebia radiata. Mäkelä MR, Lundell T, Hatakka A, et al. Fungal Biol. 117(1), 62-70, (2013)

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Biodegradation of kraft lignin by a bacterial strain Comamonas sp. B-9 isolated from eroded bamboo slips. Chen YH, Chai LY, Zhu YH, et al. J. Appl. Microbiol. 112(5), 900-6, (2012)

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Biodecolorization and biodegradation of reactive Levafix Blue E-RA granulate dye by the white rot fungus Irpex lacteus. Kalpana D, Velmurugan N, Shim JH, et al. J. Environ. Manage. 111, 142-9, (2012)

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Chlorobenzoic acid degradation by Lentinus (Panus) tigrinus: in vivo and in vitro mechanistic study-evidence for P-450 involvement in the transformation. Stella T, Covino S, Křesinová Z, et al. J. Hazard. Mater. 260, 975-83, (2013)

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Improvement of ligninolytic properties in the hyper lignin-degrading fungus Phanerochaete sordida YK-624 using a novel gene promoter. Sugiura T, Mori T, Kamei I, et al. FEMS Microbiol. Lett. 331(1), 81-8, (2012)

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Optimization of culture medium composition for manganese peroxidase and tyrosinase production during Reactive Black 5 decolourization by the yeast Trichosporon akiyoshidainum. Martorell MM, Pajot HF, Rovati JI, et al. Yeast 29(3-4), 137-44, (2012)

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Release of Pleurotus ostreatus versatile-peroxidase from Mn2+ repression enhances anthropogenic and natural substrate degradation. Salame TM, Knop D, Levinson D, et al. PLoS ONE 7(12), e52446, (2012)

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Degradation of alkylphenols by white rot fungus Irpex lacteus and its manganese peroxidase. Moon DS and Song HG Appl. Biochem. Biotechnol. 168(3), 542-9, (2012)

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Kinetic and thermodynamic characterization of the functional properties of a hybrid versatile peroxidase using isothermal titration calorimetry: Insight into manganese peroxidase activation and lignin peroxidase inhibition. Ertan H, Siddiqui KS, Muenchhoff J, et al. Biochimie 94(5), 1221-31, (2012)

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Mn(II) oxidation is the principal function of the extracellular Mn-peroxidase from Phanerochaete chrysosporium. Glenn JK, Akileswaran L, and Gold MH Arch. Biochem. Biophys. 251(2), 688-96, (1986)

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Encapsulation of ligninolytic enzymes and its application in clarification of juice. Gassara-Chatti F, Brar SK, Ajila CM, et al. Food Chem. 137(1-4), 18-24, (2013)

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Manganese peroxidase from the basidiomycete Phanerochaete chrysosporium: spectral characterization of the oxidized states and the catalytic cycle. Wariishi H, Akileswaran L, and Gold MH Biochemistry 27(14), 5365-70, (1988)

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Heterologous expression of manganese peroxidase in Aspergillus niger and its effect on phenanthrene removal from soil. Cortés-Espinosa DV, Absalón ÁE, Sanchez N, et al. J. Mol. Microbiol. Biotechnol. 21(3-4), 120-9, (2011)

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The synergistic effect on production of lignin-modifying enzymes through submerged co-cultivation of Phlebia radiata, Dichomitus squalens and Ceriporiopsis subvermispora using agricultural residues. Dong YC, Wang W, Hu ZC, et al. Bioprocess Biosyst. Eng. 35(5), 751-60, (2012)

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Degradation of endocrine disrupting chemicals by genetic transformants with two lignin degrading enzymes in Phlebia tremellosa. Kum H, Lee S, Ryu S, et al. J. Microbiol. 49(5), 824-7, (2011)

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Detoxification of azo dyes mediated by cell-free supernatant culture with manganese-dependent peroxidase activity: effect of Mn2+ concentration and H2O2 dose. Contreras E, Urra J, Vásquez C, et al. Biotechnol. Prog. 28(1), 114-20, (2012)

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Manganese peroxidase h4 isozyme mediated degradation and detoxification of triarylmethane dye malachite green: optimization of decolorization by response surface methodology. Saravanakumar T, Palvannan T, Kim DH, et al. Appl. Biochem. Biotechnol. 171(5), 1178-93, (2013)

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Kinetic properties of manganese peroxidase from the mushroom Stereum ostrea and its ability to decolorize dyes. Praveen K, Usha KY, Viswanath B, et al. J. Microbiol. Biotechnol. 22(11), 1540-8, (2012)

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Production of laccase and manganese peroxidase by Pleurotus pulmonarius in solid-state cultures and application in dye decolorization. dos Santos Bazanella GC, de Souza DF, Castoldi R, et al. Folia Microbiol. (Praha) 58(6), 641-7, (2013)

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Improvement of manganese peroxidase production by the hyper lignin-degrading fungus Phanerochaete sordida YK-624 by recombinant expression of the 5-aminolevulinic acid synthase gene. Hirai H, Misumi K, Suzuki T, et al. Curr. Microbiol. 67(6), 708-11, (2013)

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Purification and characterization of Mn-peroxidase from Musa paradisiaca (banana) stem juice. Yadav P, Singh VK, Yadav M, et al. Indian J. Biochem. Biophys. 49(1), 42-8, (2012)

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Production of ligninolytic enzymes by solid-state fermentation using Pleurotus eryngii. Akpinar M and Urek RO Prep Biochem Biotechnol. 42(6), 582-97, (2012)

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Expression of manganese peroxidase by Lentinula edodes and Lentinula boryana in solid state and submerged system fermentation. Hermann KL, Costa A, Helm CV, et al. An. Acad. Bras. Cienc. 85(3), 965-73, (2013)

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Performance of an enzymatic extract in Botrycoccus braunii cell wall disruption. Ciudad G, Rubilar O, Azócar L, et al. J. Biosci. Bioeng. 117(1), 75-80, (2014)

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