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93021013 Sigma

HeLa Cell Line human

epitheloid cervix carcinoma

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Properties

Related Categories Cell Culture, D-I, ECACC Cell Lines More...
biological source   cervix from human
growth mode   Adherent
karyotype   2n = 46, aneuploid
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Virus studies

Cell Line Origin

Human cervix epitheloid carcinoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1.3x10,000 cells/cm2 using 0.25% Trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Derived from a cervical carcinoma from a 31 year old female. This was the first aneuploid line derived from human tissue maintained in continuous cell culture. Susceptible to Poliovirus type I and adenovirus type 3. Identified as a contaminant in many other cell lines. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors

Cell analysis of the dynamics of subcellular structures has been revolutionized in the past 15 years by the development and refinement of genetically-encoded fusions between fluorescent proteins (GFP...
Keywords: Apoptosis, Cloning, Fluorescent microscopy, Gene expression, Immunocytochemistry, Immunofluorescence, Microscopy, Rearrangements, Tagged proteins, Transduction, Transfection

Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors

Autophagy, a degradative pathway that provides recycled nutrients to cells under stress, plays both protective and deleterious roles in many diseases, including cancer, neurodegeneration, and infecti...
Karyn Huryn-Selvar1, Haizhen Liu1, Janet Anderl1, Jun Ma1, Mark Santos1, Matthew Hsu1, and Luke Armstrong1

1 EMD Millipore, 28820 Single Oak Drive, Temecula, CA, USA 92590
Keywords: Autophagy, Cancer, Diseases, Flow cytometry, Fluorescent microscopy, Gene expression, Immunofluorescence, Microscopy, Tagged proteins, Transduction, Transfection

Peer-Reviewed Papers
15

References

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M7145 MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
M2279 Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
G7513 L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

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