|Related Categories||Cell Culture, ECACC Cell Lines, S-Z More...|
|biological source||blood from human|
|karyotype||Carries homogeneous chromosomal abnormality (54,X)|
|receptors||Interleukin 3 (IL-3) and Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)|
|shipped in||dry ice|
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Tumorigenic studies, analysis of IL-3, GM-CSF and EPO-receptors, study of signal transduction of haemopoietic growth factors.
STR-PCR Data: Amelogenin: X,Y
RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 2-5ng/ml Human Granulocyte Macrophage Colony Stimulating Factor (Human GM-CSF) + 10% Foetal Bovine Serum (FBS). It is recommended to add fresh GM-CSF every 48 hours.
Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.
TF-1 have been derived from a patient with erythroleukaemia. They show complete growth dependency on GM-CSF or IL-3, and carry a homogeneous chromosomal abnormality (54,X). Constitutive expression of globin genes indicate commitment of TF-1 to the erythroid lineage, although glycophorin A or carbonyl anhydrase I are not expressed. On incubation with hemin or delta-aminolevulinic acid haemoglobin synthesis was induced, whereas TPA induced differentiation into macrophage-like cells. Erythroprotein also sustains short-term growth, but does not induce differentiation.
The close relationship between DNA replication and the selection of differentiation lineages of human erythroleukemia cell lines K562, HEL, and TF1 into either erythroid or megakaryocytic lineages. Murate T, et al. Exp. Cell Res. 208(1), 35-43, (1993)
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RPMI-1640 Medium, With sodium bicarbonate, without
|S8636||Sodium pyruvate solution, 100 mM, sterile-filtered, BioReagent, suitable for cell culture|
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