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93022307 Sigma

TF1 Cell Line human

Erythroleukemic cell line from blood.



Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   blood from human
growth mode   Suspension
karyotype   Carries homogeneous chromosomal abnormality (54,X)
morphology   Not specified
products   Not specified
receptors   Interleukin 3 (IL-3) and Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C


Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.


Tumorigenic studies, analysis of IL-3, GM-CSF and EPO-receptors, study of signal transduction of haemopoietic growth factors.

Cell Line Origin

Human erythroleukaemia

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
vWA: 15,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 2-5ng/ml Human Granulocyte Macrophage Colony Stimulating Factor (Human GM-CSF) + 10% Foetal Bovine Serum (FBS). It is recommended to add fresh GM-CSF every 48 hours.

Subculture Routine

Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

TF-1 have been derived from a patient with erythroleukaemia. They show complete growth dependency on GM-CSF or IL-3, and carry a homogeneous chromosomal abnormality (54,X). Constitutive expression of globin genes indicate commitment of TF-1 to the erythroid lineage, although glycophorin A or carbonyl anhydrase I are not expressed. On incubation with hemin or delta-aminolevulinic acid haemoglobin synthesis was induced, whereas TPA induced differentiation into macrophage-like cells. Erythroprotein also sustains short-term growth, but does not induce differentiation.

Price and Availability

Flow Cytometry Instruments
Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers


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R0883 RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
G7513 L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
S8636 Sodium pyruvate solution, 100 mM, sterile-filtered, BioReagent, suitable for cell culture

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