Properties
| Related Categories | Cell Culture, ECACC Cell Lines, O-R More... |
| biological source | embryo from rat |
| growth mode | Adherent |
| karyotype | Near diploid |
| morphology | Fibroblast |
| shipped in | dry ice |
| storage temp. | −196°C |
Description
General description
For complete product information, please see R9
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Cell Line Description
The R74 line was derived by co-cultivation of 18 day-old Lewis rat embryonic fibroblasts with cells of a chicken positive for Rous sarcoma virus-33 (RSV-33). The R9 cell population is clearly distinguishable from RT1 and R74 cell lines because it contains flat polygonal and triangular refractile cells with a tendency to round up. It is also tumourigenic in chickens and rats.
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