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93289 Sigma

TRI Reagent®

for DNA, RNA and protein isolation

Synonym: TRI Reagent® RNA Isolation Reagent

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Properties

Related Categories DNA & RNA Purification, Molecular Biology, RNA Purification Kits, Reagents
grade   for DNA, RNA and protein isolation
usage   1 mL sufficient for 107 cells
  1 mL sufficient for 100 mg tissue
impurities   phenol

Description

Application

For processing tissues, cells cultured in monolayer, or cell pellets.

TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

Features and Benefits

• Easily scalable RNA isolation
• Works with many sources: human, plant, yeast, bacterial, or viral
• Better yields than traditional guanidine thiocyanate/cesium chloride methods

General description

This improved version of the single-step total RNA isolation reagent developed by Chomczynski allows a quick, economical, and efficient isolation of total RNA from different biological samples. This product is pure (A260/A280 ~1.8) and can be directly used in Northern blotting. Simultaneous isolation of DNA, RNA and proteins is also possible.
1 mL is sufficient for the extraction of 100 mg tissue or 107 cells.

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Price and Availability


NAP and qPCR Reagents from Roche

Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

Signal word 
Danger
Supplemental Hazard Statements 
Contact with acids liberates very toxic gas.
RIDADR 
UN 2922 6.1(8) / PGIII
WGK Germany 
3
Flash Point(F) 
174.2 °F
Flash Point(C) 
79 °C

Documents

Certificate of Analysis

Protocols & Articles

Articles

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

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T3934 TRI Reagent®, LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid.
T9424 TRI Reagent®, For processing tissues, cells cultured in monolayer or cell pellets
T3809 TRI Reagent®, BD, For processing whole blood, plasma, or serum.

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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