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93309 Sigma

TRIS borate – EDTA buffer substance

for Northern and Southern blotting, powder

Synonym: TBE buffer dry blend

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Properties

grade   for molecular biology
product line   BioReagent
form   powder
quality   5× concentrate
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
color   white
storage temp.   room temp

Description

Components

The final 5x TBE liquid contains 0.445M TRIS borate (pH 8.3) and 0.01M EDTA in water.

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This powder should be reconstituted in molecular biology grade water before use.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles

Articles

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

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