93337 Sigma

Trizma® acetate

BioUltra, ≥99.0% (NT)

Synonym: TRIS acetate salt, Tris(hydroxymethyl)aminomethane acetate salt

  • CAS Number 6850-28-8

  • Linear Formula NH2C(CH2OH)3 · CH3COOH

  • Molecular Weight 181.19

  •  Beilstein Registry Number 3702918

  •  EC Number 229-939-6

  •  MDL number MFCD00038951

  •  PubChem Substance ID 57653535



Related Categories Alphabetical, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers, Zwitterionic More...
product line   BioUltra
assay   ≥99.0% (NT)
impurities   insoluble matter, passes filter test
ign. residue   ≤0.2%
loss   ≤0.2% loss on drying, 20 °C (HV)
pH   6.0-7.0 (25 °C, 0.5 M in H2O)
solubility   H2O: soluble0.5 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   0.5 M in H2O
UV absorption   λ: 260 nm Amax: 0.06
  λ: 280 nm Amax: 0.05


Other Notes

Recommended buffer for maximum sensitivity of ATP assays with firefly luciferase; Assay of glutamate binding


Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Suggested Laboratory Gloves

Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers


Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?