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93352 Sigma

Trizma® base

≥99.0% (T)

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein Registry Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

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Properties

description   aminopeptidase substrate
InChI Key   LENZDBCJOHFCAS-UHFFFAOYSA-N
product line   BioChemika
assay   ≥99.0% (T)
form   crystalline
loss   ≤1% loss on drying, 110 °C
color   white
pH   10.5-12.0(4 m in water, 25 °C)
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
  168-172 °C
solubility   H2O: soluble1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg

Description

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Application

Trizma® base was used as buffer for the following studies:
• Electrophoretic transfer for the specific identification of isozymes of starch debranching enzyme, α-amylase and 9-amylase.
• Electrophoretic separation of lipoproteins in polyacrylamide gels.
• Preparation of TRIS buffer having pH 8.
It may be used to compose DN buffer for DNA nick-end labeling of tissue sections.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Tris Always Available
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TY2900000
Protocols & Articles
Peer-Reviewed Papers
15

References

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