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93426 Sigma

Triton X-100

BioXtra, for molecular biology

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Detergents, Detergents N to Z,
grade   for molecular biology
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
product line   BioXtra
form   liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
aggregation number   100-155
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.2%
CMC   0.2-0.9 mM(20-25°C)
transition temp   cloud point 65 °C
  pour point ~7 °C
solubility   H2O: soluble0.005 M at 20 °C, clear, colorless
density   1.065 g/mL at 20 °C
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤500 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤500 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
HLB   13.5
storage temp.   2-8°C

Description

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Principle

Triton X-100 compromises the integrity of the lipid bilayer by disrupting the polar head group on the hydrogen bonding. The detergent monomer inserts itself into the lipid bilayer resulting in over-permeabilization and disruption of the cell structure.

Application

Suitable for extraction of membrane-bound proteins

General description

Triton X-100 is a non-ionic surfactant that aids the recovery of membrane-bound proteins under mild non-denaturing conditions.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3
RTECS 
MD0907700
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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