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93426 Sigma

Triton X-100

BioXtra, for molecular biology

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Detergents, Detergents N to Z,
grade   for molecular biology
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
product line   BioXtra
form   liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
aggregation number   100-155
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.2%
CMC   0.2-0.9 mM(20-25°C)
transition temp   cloud point 65 °C
  pour point ~7 °C
solubility   H2O: 0.005 M at 20 °C clear, colorless
density   1.065 g/mL at 20 °C
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤500 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤500 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
HLB   13.5
storage temp.   2-8°C

Description

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Principle

Triton X-100 compromises the integrity of the lipid bilayer by disrupting the polar head group on the hydrogen bonding. The detergent monomer inserts itself into the lipid bilayer resulting in over-permeabilization and disruption of the cell structure.

Application

Suitable for extraction of membrane-bound proteins

General description

Triton X-100 is a non-ionic surfactant that aids the recovery of membrane-bound proteins under mild non-denaturing conditions.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3
RTECS 
MD0907700
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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