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  • 96632 - Bis(2,2′-bipyridine)-4,4′-dicarboxybipyridine-ruthenium di(N-succinimidyl ester) bis(hexafluorophosphate)

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96632 Sigma

Bis(2,2′-bipyridine)-4,4′-dicarboxybipyridine-ruthenium di(N-succinimidyl ester) bis(hexafluorophosphate)

BioReagent, suitable for fluorescence

Synonym: Ru(bpy)2(dcbpy-di O-Su-ester)(PF6)2

  • CAS Number 160525-49-5

  • Empirical Formula (Hill Notation) C40H30F12N8O8P2Ru

  • Molecular Weight 1141.71

  •  MDL number MFCD02683416

  •  PubChem Substance ID 57653771

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains, Luminescent Compounds/Detection, Metal Complexes,
InChI Key   HIPJTYPALXAMBX-UHFFFAOYSA-N
product line   BioReagent
fluorescence   λex 458 nm; λem 647 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
shipped in   dry ice
storage temp.   −20°C

Description

Application

Ruthenium-ligand belonging to a new class of long-lived fluorophores for protein hydrodynamics.

Features and Benefits

Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds. These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes’ shifts. In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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