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96827 Sigma

Renaturation Basic Kit for Proteins

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Description

Components

The kit contains 50 components with 10 mL each. Most components can be ordered separately as 100 mL packages.

Features and Benefits

Advantages
• Comprehensive, efficient screening
• Stringent purity standards
• Prefabricated reagents, application sheets, and observation sheets
• Low incidence of undesirable solubility effects

General description

The Renaturation Basic Kit for Proteins is a rapid empirical screening method used to determine the best conditions for the renaturation of proteins which have been solubilized from inclusion bodies. Physical parameters, such as pH, redox potential, ionic strength, low-molecular weight additives and temperature, may have an effect on the efficiency of in vitro renaturation processes. Screening with this kit is an easy method to find renaturation conditions in a defined pH range of 6.5 to 8.5, defined redox potential and ionic strength conditions, as well as the effect of a sulfobetaine [3-(1-1-pyridinio)-1-propanesulfonate, included in the kit]. Other parameters (e.g., concentration of the solubilized protein, temperature and additional additives) can easily be added to the basic screening conditions of this kit.

Price and Availability


Amplified Detection

Kit component also available separately

Description

Product #

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MES pH 6.5; 0.05M – Sodium chloride 0.1M – PPS 1M solution SDS 83047
MES pH 6.5; 0.05M – Sodium chloride 0.2M solution SDS 82953
MES pH 6.5; 0.05M – Sodium chloride 0.2M – PPS 1M solution SDS 72905
MES pH 6.5; 0.05M – DTT 0.001M Solution solution SDS 73021
MES pH 6.5; 0.05M – DTT 0.001M – PPS 1M solution SDS 71023
MES pH 6.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M solution SDS 93038
MES pH 6.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M – PPS 1M solution SDS 85312
MES pH 6.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M solution SDS 83809
MES pH 6.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M – PPS 1M solution SDS 73194
MES pH 6.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution SDS 72476
MES pH 6.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M , PPS 1M solution SDS 77809
MES pH 6.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution SDS 78569
MES pH 6.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution SDS 72914
MES pH 6.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution SDS 78582
HEPES Sodium salt pH 7.5; 0.05M solution SDS 94717
HEPES Sodium salt pH 7.5; 0.05M – PPS 1M solution SDS 78593
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M solution SDS 87062
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – PPS 1M solution SDS 78579
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M solution SDS 87605
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – PPS 1M solution SDS 72906
HEPES Sodium salt pH 7.5; 0.05M – DTT 0.001M solution SDS 78152
HEPES Sodium salt pH 7.5; 0.05M – DTT 0.001M – PPS 1M solution SDS 72824
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M solution SDS 72588
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M – PPS 1M solution SDS 76983
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M solution SDS 73193
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M – PPS 1M solution SDS 80293
HEPES Sodium salt pH 7.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution SDS 80292
HEPES Sodium salt pH 7.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution SDS 76951
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution SDS 83068
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution SDS 83796
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution SDS 80409
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M solution SDS 75837
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – PPS 1M solution SDS 91810
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M solution SDS 82959
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – PPS 1M solution SDS 83943
TRIS hydrochloride pH 8.5; 0.05M – DTT 0.001M solution SDS 82305
TRIS hydrochloride pH 8.5; 0.05M – DTT 0.001M – PPS 1M solution SDS 70603
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M solution SDS 75812
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M – PPS 1M solution SDS 81220
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M solution SDS 72259
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M – PPS 1M solution SDS 72991
TRIS hydrochloride pH 8.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution SDS 78217
TRIS hydrochloride pH 8.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution SDS 95739
TRIS hydrochloride pH 8.5; 0.05M –Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution SDS 82972
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution SDS 72403
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution SDS 85323
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution SDS 93039
MES pH 6.5; 0.05M – Sodium chloride 0.1M solution SDS 72903
HEPES Sodium salt pH 7.5; 0.05M – 0.1M Sodium chloride, L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution SDS 88503
MES pH 6.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution SDS 94718
Safety & Documentation

Safety Information

RIDADR 
UN 3316 9

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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