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99072804 Sigma

T-3-CI-2-0.fl cell line



biological source   Spleen from mouse
growth mode   Suspension
karyotype   Not specified
morphology   Lymphoblastoid-like
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C


Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.


Study of erythroid differentiation

Cell Line Origin

Mouse friend leukemia lymphoblast

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5%CO2; 37ºC

Cell Line Description

T-3-Cl-2-0.fl is a clone of T-3-Cl-2, which originated from a splenic lesion of Friend virus-induced leukemia in a DD mouse. The cell line can be induced to differentiate into erythroblastoid cells with 1.5% DMSO. T-3-Cl-2-0.fl has been eradicated from mycoplasma using MC-210 by the originator.

Price and Availability

Cultivate Consistency
Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Protocols & Articles
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