Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat

Other Notes

Antibody adsorbed with human IgG and rat serum proteins.

Immunogen

Purified mouse IgG

Preparation Note

Adsorbed to reduce background staining with human or rat samples.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ with 50% glycerol as stabilizer and 15 mM sodium azide.

Application

Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.¹

Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is suitable for use in western blot at 1:1000 dilution using human airway epithelial cells or using whole-cell extracts of rat tubal tissues . The antibody can also be used for immunohistochemistry (1:10 using formalin-fixed, paraffin-embedded sections).

Goat anti-mouse Fab specific alkaline phosphatase antibody was used in direct ELISA assays to identify Mabs with reactivity to mouse thyroglobulin.Goat anti-mouse Fab specific alkaline phosphatase antibody was used to analyze serum Ig titers by His Sorb ELISA at 1:10000 at room temperature for two hours. Antibodies were diluted in PBS plus 0.2% BSA to reduce background.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases ². Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice ³. Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is detects mouse IgG (whole molecule). When tested by ELISA, the product reacts with the Fab fragment of mouse IgG but does not recognize the Fc fragment of mouse IgG, human IgG, IgA, IgM or rat IgG.