Monoclonal Anti-Rabbit Immunoglobulins−Alkaline Phosphatase antibody produced in mouse
clone RG-16, purified immunoglobulin, buffered aqueous glycerol solution
DOWNLOAD MSDS (PDF)Synonym: Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
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Properties
| Related Categories | Alkaline Phosphatase, Alkaline Phosphatase Conjugates, Alphabetical Index, Antibodies, Antibody Conjugates, |
| antibody form | purified immunoglobulin |
| clone | RG-16, monoclonal |
| form | buffered aqueous glycerol solution |
| does not react with | guinea pig, turkey, rat, goat, canine, feline, bovine, sheep, chicken, horse, human, pig |
| application(s) | direct ELISA: 1:40,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody | |
| western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well) |
Description
General description
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
Specificity
Binds only rabbit Igs. Does not react with reduced rabbit Igs.
Physical form
Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 0.1% bovine serum albumin, 50% glycerol and 15 mM sodium azide
Application
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.1
Price and Availability
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