|Related Categories||Alkaline Phosphatase, Alkaline Phosphatase Conjugates, Alphabetical Index, Antibodies, Antibody Conjugates,|
|antibody form||purified immunoglobulin|
|form||buffered aqueous glycerol solution|
|does not react with||guinea pig, turkey, rat, goat, canine, feline, bovine, sheep, chicken, horse, human, pig|
|application(s)||direct ELISA: 1:40,000|
|immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody|
|western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well)|
|shipped in||wet ice|
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
Binds only rabbit Igs. Does not react with reduced rabbit Igs.
Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 0.1% bovine serum albumin, 50% glycerol and 15 mM sodium azide
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.1
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
1. Vaccination of Rabbits with an Alkylated Toxoid Rapidly Elicits Potent Neutralizing Antibodies against Botulinum Neurotoxin Serotype B 17:930-936. Held, D. Clin. Vaccine Immunol. 17, 930-936, (2010)
Polymerization of nonfilamentous actin into microfilaments is an important process for porcine oocyte maturation and early embryo development. Abeydeera, L., et al. Biol. Reprod. 62, 1177-1183, (2000)
The Immune Evasion Paradox: Immunoevasins of Murine Cytomegalovirus Enhance Priming of CD8 T Cells by Prevention of Negative Feed-Back Regulation. Simon, C.O., et al. J. Virol. 23, 11637-11650, (2008)
Transactivation Of Cellular Genes Involved In Nucleotide Metabolism By The Regulatory IE1 Protein Of Murine Cytomegalovirus Is Not Critical For Viral Replicative Fitness In Quiescent Cells And Host Tissues. Wilhelmi, V., et al. J. Virol. Meth. 82, 9900-16, (2008)
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