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A2929 Sigma

Agarose

For pulsed field electrophoresis running gel, DNase, RNase, NICKase, none detected

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Properties

Related Categories Agarose, Agarose Gel, Core Bioreagents, Molecular Biology, Nucleic Acid Electrophoresis,
impurities   ≤10% water
EEO   0.04-0.10
transition temp   gel point 38-43 °C
anion traces   sulfate (SO42-): ≤0.20%
foreign activity   DNase, RNase, NICKase, none detected

Description

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Especially formulated for fast resolution of large DNA (10-2000 ·kb) by pulsed-field gel electrophoresis (PFGE) or conventional electrophoresis. Pulsed-field gel electrophoresis was developed in 1984 to deal with "reptation" of large DNA′s whose size surpass the limit of resolution and all migrate at the same rate. Alternately pulsed, orthogonally oriented electric fields are applied across the gel. Every time the direction of the electric field changes, the large DNA′s can no longer migrate until they become reoriented along the new electric field. The time required for reorientation is dependent on the size of the DNA, therefore the DNA is fractionated by size.

Features and Benefits

• Low EEO allows for increased voltages and more rapid DNA migration
• Greater gel strength allows for stability of lower concentration gels leading to faster DNA migration
• Gels exhibit low background fluorescence using ethidium bromide staining

Packaging

25, 100, 250 g in poly bottle

Specificity

Suitable for the separation of high molecular weight DNA. Gels are easy to handle and give faster separation and better resolution of high molecular weight DNA by field inversion electrophoresis than our routine-use agarose.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



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Safety & Documentation

Protocols & Articles

Related Content

Agarose Selection Guide

From our library of Related Content, Sigma-Aldrich presents Agarose Selection Guide
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Peer-Reviewed Papers

References

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Towards the N-terminal acetylome: an N-terminal acetylated peptide enrichment method using CNBr-activated sepharose resin. Zhang X and Højrup P Methods Mol. Biol. 981, 47-56, (2013)

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Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR. Leng X and Yang CJ Methods Mol. Biol. 949, 413-22, (2013)

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The Pbx interaction motif of Hoxa1 is essential for its oncogenic activity. Delval, S., et al. PLoS ONE 6(9), e25247, (2011)

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Protein adsorption to poly(ethylenimine)-modified Sepharose FF: III. Comparison between different proteins. Hong Y, Liu N, Wei W, et al. J. Chromatogr. A 1342, 30-6, (2014)

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Multifunctional three-dimensional macroporous nanoelectronic networks for smart materials. Liu J, Xie C, Dai X, et al. Proc. Natl. Acad. Sci. U. S. A. 110(17), 6694-9, (2013)

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Glycogen synthase kinase 3beta-mediated serine phosphorylation of the human glucocorticoid receptor redirects gene expression profiles. Galliher-Beckley AJ Mol. Cell. Biol. 28(24), 7309-22, (2008)

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Combination of fibrin-agarose hydrogels and adipose-derived mesenchymal stem cells for peripheral nerve regeneration. Carriel V, Garrido-Gómez J, Hernández-Cortés P, et al. J. Neural Eng. 10(2), 026022, (2013)

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Dynamic integration of subplate neurons into the cortical barrel field circuitry during postnatal development in the Golli-tau-eGFP (GTE) mouse. Piñon MC J. Physiol. 587(Pt 9), 1903-15, (2009)

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Agarose overlay selectively improves macrocolony formation and radiosensitivity assessment in primary fibroblasts. Chandna S, Dagur RS, Mathur A, et al. Int. J. Radiat. Biol. 90(5), 401-6, (2014)

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Residues in the 11 A channel of histone deacetylase 1 promote catalytic activity: implications for designing isoform-selective histone deacetylase inhibitors. Weerasinghe SV J. Med. Chem. 51(18), 5542-51, (2008)

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Temporal effect of inertial cavitation with and without microbubbles on surface deformation of agarose S gel in the presence of 1-MHz focused ultrasound. Tomita Y, Matsuura T, and Kodama T Ultrasonics 55, 1-5, (2015)

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Structural basis for Pan3 binding to Pan2 and its function in mRNA recruitment and deadenylation. Wolf J, Valkov E, Allen MD, et al. EMBO J. 33(14), 1514-26, (2014)

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Tailor-made cell patterning using a near-infrared-responsive composite gel composed of agarose and carbon nanotubes. Koga H, Sada T, Fujigaya T, et al. Biofabrication 5(1), 015010, (2013)

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Modification of agarose with carboxylation and grafting dopamine for promotion of its cell-adhesiveness. Su Y, Chu B, Gao Y, et al. Carbohydr. Polym. 92(2), 2245-51, (2013)

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Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts. McCoy F, Darbandi R, Chen SI, et al. J. Biol. Chem. 288(13), 8838-48, (2013)

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Competitive stem cell recruitment by multiple cytotactic cues. Mendelson A, Cheung Yk, Paluch K, et al. Lab Chip 13(6), 1156-64, (2013)

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In-gel expression and in situ immobilization of proteins for generation of three dimensional protein arrays in a hydrogel matrix. Byun JY, Lee KH, Lee KY, et al. Lab Chip 13(5), 886-91, (2013)

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Regeneration of viable oil palm plants from protoplasts by optimizing media components, growth regulators and cultivation procedures. Masani MY, Noll G, Parveez GK, et al. Plant Sci. 210, 118-27, (2013)

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Development of MRI phantom equivalent to human tissues for 3.0-T MRI. Hattori K, Ikemoto Y, Takao W, et al. Med. Phys. 40(3), 032303, (2013)

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A conserved acidic patch in the Myb domain is required for activation of an endogenous target gene and for chromatin binding. Ko ER Mol. Cancer 7, 77, (2008)

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Co-immobilization of fungal endo-xylanase and α-L-arabinofuranosidase in glyoxyl agarose for improved hydrolysis of arabinoxylan. Damásio AR, Pessela BC, da Silva TM, et al. J. Biochem. 154(3), 275-80, (2013)

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Conformational and thermal stability improvements for the large-scale production of yeast-derived rabbit hemorrhagic disease virus-like particles as multipurpose vaccine. Fernández E, Toledo JR, Méndez L, et al. PLoS ONE 8(2), e56417, (2013)

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Integrative refolding and purification of histidine-tagged protein by like-charge facilitated refolding and metal-chelate affinity adsorption. Liu H, Du WJ, Dong XY, et al. J. Chromatogr. A 1344, 59-65, (2014)

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Distinct population of highly malignant cells in a head and neck squamous cell carcinoma cell line established by xenograft model. Chen CY J. Biomed. Sci. 16, 100, (2009)

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An extra peptide within the catalytic module of a β-agarase affects the agarose degradation pattern. Han WJ, Gu JY, Liu HH, et al. J. Biol. Chem. 288(13), 9519-31, (2013)

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Binding analysis between L-histidine immobilized and oligonucleotides by SPR and NMR. Cruz C, Santos SD, Cabrita EJ, et al. Int. J. Biol. Macromol. 56, 175-80, (2013)

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An ectotherm homologue of human predicted gene NAT16 encodes histidine N-acetyltransferase responsible for Nα-acetylhistidine synthesis. Yamada S and Arikawa S Biochim. Biophys. Acta 1840(1), 434-42, (2014)

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Purification of human immunoglobulin G autoantibodies to tumor necrosis factor using affinity chromatography and magnetic separation. Sennikov SV, Golikova EA, Kireev FD, et al. J. Immunol. Methods 390(1-2), 92-8, (2013)

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Simple generation of neurons from human embryonic stem cells using agarose multiwell dishes. Birenboim R, Markus A, and Goldstein RS J. Neurosci. Methods 214(1), 9-14, (2013)

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Mousepox detected in a research facility: case report and failure of mouse antibody production testing to identify Ectromelia virus in contaminated mouse serum. Labelle P Comp. Med. 59(2), 180-6, (2009)

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Cesium accumulation of Rhodococcus erythropolis CS98 strain immobilized in hydrogel matrices. Takei T, Yamasaki M, and Yoshida M J. Biosci. Bioeng. 117(4), 497-500, (2014)

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Accuracy of iodine removal using dual-energy CT with or without a tin filter: an experimental phantom study. Kawai T, Takeuchi M, Hara M, et al. Acta radiol. 54(8), 954-60, (2013)

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Trypanosoma cruzi chemical proteomics using immobilized benznidazole. Trochine A, Alvarez G, Corre S, et al. Exp. Parasitol. 140, 33-8, (2014)

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Agarose gel tailored calcium carbonate nanoparticles-synthesis and biocompatibility evaluation. Biradar S, Goornavar V, Periyakaruppan A, et al. J. Nanosci. Nanotechnol. 14(6), 4257-63, (2014)

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Determination of leakage from antibody adsorbent: composition analysis and pH effect. Zhao L, Liu Y, Wang Y, et al. Biomed. Chromatogr. 27(9), 1089-91, (2013)

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Hydrolysis of chickpea proteins with Flavourzyme immobilized on glyoxyl-agarose gels improves functional properties. del Mar Yust M, del Carmen Millán-Linares M, Alcaide-Hidalgo JM, et al. Food Sci. Technol. Int. 19(3), 217-23, (2013)

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3D arrays for high throughput assay of cell migration and electrotaxis. Zhao S, Gao R, Devreotes PN, et al. Cell Biol. Int. 37(9), 995-1002, (2013)

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Removable silicon insertion stiffeners for neural probes using polyethylene glycol as a biodissolvable adhesive. Felix S, Shah K, George D, et al. Conf. Proc. IEEE Eng. Med. Biol. Soc. 2012, 871-4, (2012)

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RegBook 1 (1), 203:C

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