|Related Categories||Agarose, Agarose Gel, Core Bioreagents, Molecular Biology, Nucleic Acid Electrophoresis,|
|transition temp||gel point 38-43 °C|
|anion traces||sulfate (SO42-): ≤0.20%|
|foreign activity||DNase, RNase, NICKase, none detected|
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Especially formulated for fast resolution of large DNA (10-2000 ·kb) by pulsed-field gel electrophoresis (PFGE) or conventional electrophoresis. Pulsed-field gel electrophoresis was developed in 1984 to deal with "reptation" of large DNA′s whose size surpass the limit of resolution and all migrate at the same rate. Alternately pulsed, orthogonally oriented electric fields are applied across the gel. Every time the direction of the electric field changes, the large DNA′s can no longer migrate until they become reoriented along the new electric field. The time required for reorientation is dependent on the size of the DNA, therefore the DNA is fractionated by size.
• Low EEO allows for increased voltages and more rapid DNA migration
• Greater gel strength allows for stability of lower concentration gels leading to faster DNA migration
• Gels exhibit low background fluorescence using ethidium bromide staining
25, 100, 250 g in poly bottle
Suitable for the separation of high molecular weight DNA. Gels are easy to handle and give faster separation and better resolution of high molecular weight DNA by field inversion electrophoresis than our routine-use agarose.
Certificate of Analysis
Certificate of Origin
From our library of Related Content, Sigma-Aldrich presents Agarose Selection Guide
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation
Residues in the 11 A channel of histone deacetylase 1 promote catalytic activity: implications for designing isoform-selective histone deacetylase inhibitors. Weerasinghe SV J. Med. Chem. 51(18), 5542-51, (2008)
Mousepox detected in a research facility: case report and failure of mouse antibody production testing to identify Ectromelia virus in contaminated mouse serum. Labelle P Comp. Med. 59(2), 180-6, (2009)
Conformational and thermal stability improvements for the large-scale production of yeast-derived rabbit hemorrhagic disease virus-like particles as multipurpose vaccine. Fernández E, Toledo JR, Méndez L, et al. PLoS ONE 8(2), e56417, (2013)
A biomimetic Protein G affinity adsorbent: an Ugi ligand for immunoglobulins and Fab fragments based on the third IgG-binding domain of Protein G. El Khoury G and Lowe CR J. Mol. Recognit. 26(4), 190-200, (2013)
Magnetic resonance imaging (MRI) markers for MRI-guided high-dose-rate brachytherapy: novel marker-flange for cervical cancer and marker catheters for prostate cancer. Schindel J, Muruganandham M, Pigge FC, et al. Int. J. Radiat. Oncol. Biol. Phys. 86(2), 387-93, (2013)
A non-exothermic cell-embedding tissue-mimicking material for studies of ultrasound-induced hyperthermia and drug release. Mylonopoulou E, Bazán-Peregrino M, Arvanitis CD, et al. Int. J. Hyperthermia 29(2), 133-44, (2013)
Purification of human immunoglobulin G autoantibodies to tumor necrosis factor using affinity chromatography and magnetic separation. Sennikov SV, Golikova EA, Kireev FD, et al. J. Immunol. Methods 390(1-2), 92-8, (2013)
Removable silicon insertion stiffeners for neural probes using polyethylene glycol as a biodissolvable adhesive. Felix S, Shah K, George D, et al. Conf. Proc. IEEE Eng. Med. Biol. Soc. 2012, 871-4, (2012)
Cat and dog primordial follicles enclosed in ovarian cortex sustain viability after in vitro culture on agarose gel in a protein-free medium. Fujihara M, Comizzoli P, Wildt DE, et al. Reprod. Domest. Anim. 47 Suppl 6, 102-8, (2012)
Integrated system for temperature-controlled fast protein liquid chromatography comprising improved copolymer modified beaded agarose adsorbents and a travelling cooling zone reactor arrangement. Müller TK, Cao P, Ewert S, et al. J. Chromatogr. A 1285, 97-109, (2013)
Hydrolysis of chickpea proteins with Flavourzyme immobilized on glyoxyl-agarose gels improves functional properties. del Mar Yust M, del Carmen Millán-Linares M, Alcaide-Hidalgo JM, et al. Food Sci. Technol. Int. 19(3), 217-23, (2013)
The use of agarose microwells for scalable embryoid body formation and cardiac differentiation of human and murine pluripotent stem cells. Dahlmann J, Kensah G, Kempf H, et al. Biomaterials 34(10), 2463-71, (2013)
Combination of fibrin-agarose hydrogels and adipose-derived mesenchymal stem cells for peripheral nerve regeneration. Carriel V, Garrido-Gómez J, Hernández-Cortés P, et al. J. Neural Eng. 10(2), 026022, (2013)
WFDC1/ps20 is a novel innate immunomodulatory signature protein of human immunodeficiency virus (HIV)-permissive CD4+ CD45RO+ memory T cells that promotes infection by upregulating CD54 integrin expression and is elevated in HIV type 1 infection. R. Alvarez et al J. Virol. 82, 471-86, (2008)
RegBook 1 (1), 203:C
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Need larger quantities for your development, manufacturing or research applications?