Anti-Mouse IgA (α-chain specific)−Peroxidase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Properties

Related Categories	Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies, Primary Antibodies, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies More...
antibody form	affinity isolated antibody
clone	polyclonal
form	buffered aqueous solution
application(s)	direct ELISA: 1:10,000
shipped in	dry ice
storage temp.	−20°C

Description

Immunogen

Purified mouse IgA

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p 215 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal.

Application

Anti-mouse IgA (α-chain specific)-peroxidase antibody can be used in modified indirect DIBA (dot- immunobinding assay) ¹.

ELISAS were performed using HRP conjugated gaot anti-mouse IgA as the secondary antibody at a dilution of 1:10000 for a 1 hour incubation at 37 degrees to detect IgA levels in mouse fecal extracts.

General description

IgA antibody plays an essential role in mucosal immunity and binds with immunogens/pathogens to restrict them from entering the mucosal membrane². Anti-mouse IgA (α-chain specific)-peroxidase antibody can be used for determining the secretory IgA (sIgA) in intestinal fluid³. It can also be used in direct ELISA. Goat anti-mouse IgA (α-chain specific)-peroxidase antibody reacts specifically with mouse IgA.

Price and Availability

Related Papers

2. Intestinal IgA synthesis: regulation of front-line body defences. Fagarasan S, Honjo T Nat. Rev. Immunol. 3(1), 63-72, (2003)

Documents

Certificate of Analysis

A4789 - Datasheet (55 KB)

Antibody Explorer

Safety Information

WGK Germany

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Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Papers

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1. The Immune Response in Mice Immunized with Lactobacillus acidophilus LF221 - A Potential Probiotic Strain. Irena Rogelj Food Tech. Biotechnol. 37(3), 153-158, (1999)

2. Intestinal IgA synthesis: regulation of front-line body defences. Fagarasan S, Honjo T Nat. Rev. Immunol. 3(1), 63-72, (2003)

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3. Saccharomyces boulardii stimulates sIgA production and the phagocytic system of gnotobiotic mice. Cara, D., et al. J. Appl. Microbiol. 89, 404-414, (2000)

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Transport pathways for clearance of human Alzheimer’s amyloid β-peptide and apolipoproteins E and J in the mouse central nervous system. Bedi, G., et al. J. Cereb. Blood Flow Metab. 27, 909-918, (2007)

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Intra nasal administration of poly-lactic acid microsphere co-encapsulated Yersinia pestis subunits confers protection from pneumonic plague in the mouse. Alpar, H., et al. Vaccine 16, 698-707, (1998)

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Oral Immunization with Recombinant Norwalk Virus-Like Particles Induces a Systemic and Mucosal Immune Response in Mice Ball, J. J. Virol. 72, 1345-1353, (1998)

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In situ gelling nasal inserts for influenza vaccine delivery. Bertram, U., et al. Drug Devel. Ind. Pharm. 36, 581-93, (2010)