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Crude preparation containing aconitase activity
One unit will convert 1.0 μmole of citrate (via cis-aconitate) to isocitrate per min at pH 7.4 at 25 °C.
Aconitase may be usefull in diabetes research 1as well as to study structural conservation between iron-responsive element binding proteins (IRE-BP) and isomerases 2.
Aconitase catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle. Aconitase is inhibited by cyanide, sulfide and copper and mercury at low concentrations. It is competitively inhibited by trans-aconitate. Aconitase contains an internal Fe-S cluster which is responsible for its activity.
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ammonium sulfate suspension, ≥100 units/mg protein
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[Effect of combined therapy with melaxen on lipid peroxidation rate and citrate content in blood serum of patients with drug-induced hepatitis diagnosis]. Popov SS, Shul'gin KK, Pashkov AN, et al. Eksp. Klin. Farmakol. 75(7), 32-5, (2012)
Decreased glycolytic and tricarboxylic acid cycle intermediates coincide with peripheral nervous system oxidative stress in a murine model of type 2 diabetes. Hinder LM, Vivekanandan-Giri A, McLean LL, et al. J. Endocrinol. 216(1), 1-11, (2013)
Angiotensin receptor blockade recovers hepatic UCP2 expression and aconitase and SDH activities and ameliorates hepatic oxidative damage in insulin resistant rats. Montez P, Vázquez-Medina JP, Rodríguez R, et al. Endocrinology 153(12), 5746-59, (2012)
The fungal ?-aminoadipate pathway for lysine biosynthesis requires two enzymes of the aconitase family for the isomerization of homocitrate to homoisocitrate. Fazius, F., et al. Mol. Microbiol. 86, 1508-1530, (2012)
Identification of nonferritin mitochondrial iron deposits in a mouse model of Friedreich ataxia. Whitnall M, Suryo Rahmanto Y, Huang ML, et al. Proc. Natl. Acad. Sci. U. S. A. 109(50), 20590-5, (2012)
Skeletal muscle uncoupling-induced longevity in mice is linked to increased substrate metabolism and induction of the endogenous antioxidant defense system. Keipert S, Ost M, Chadt A, et al. Am. J. Physiol. Endocrinol. Metab. 304(5), E495-506, (2013)
Pro-inflammatory cytokines modulate iron regulatory protein 1 expression and iron transportation through reactive oxygen/nitrogen species production in ventral mesencephalic neurons. Wang J, Song N, Jiang H, et al. Biochim. Biophys. Acta 1832(5), 618-25, (2013)
The bifunctional iron-responsive element binding protein/cytosolic aconitase: the role of active-site residues in ligand binding and regulation. Philpott CC, Klausner RD, and Rouault TA Proc. Natl. Acad. Sci. U. S. A. 91(15), 7321-5, (1994)
Cloning of the cDNA encoding an RNA regulatory protein--the human iron-responsive element-binding protein. Rouault TA, Tang CK, Kaptain S, et al. Proc. Natl. Acad. Sci. U. S. A. 87(20), 7958-62, (1990)
Disturbance of redox homeostasis by ornithine and homocitrulline in rat cerebellum: a possible mechanism of cerebellar dysfunction in HHH syndrome. Zanatta A, Viegas CM, Tonin AM, et al. Life Sci. 93(4), 161-8, (2013)
Inhibition of protein kinase C βII isoform rescues glucose toxicity-induced cardiomyocyte contractile dysfunction: role of mitochondria. Wang Z, Zhang Y, Guo J, et al. Life Sci. 93(2-3), 116-24, (2013)
Carbonylation of mitochondrial aconitase with 4-hydroxy-2-(E)-nonenal: localization and relative reactivity of addition sites. Liu Q, Simpson DC, and Gronert S Biochim. Biophys. Acta 1834(6), 1144-54, (2013)
Mitochondria-targeted antioxidant promotes recovery of skeletal muscle mitochondrial function after burn trauma assessed by in vivo 31P nuclear magnetic resonance and electron paramagnetic resonance spectroscopy. Righi V, Constantinou C, Mintzopoulos D, et al. FASEB J. 27(6), 2521-30, (2013)
Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf]. Martínez-Cuenca MR, Iglesias DJ, Talón M, et al. Tree Physiol. 33(3), 320-9, (2013)
Cardiac-specific overexpression of metallothionein rescues against cigarette smoking exposure-induced myocardial contractile and mitochondrial damage. Hu N, Han X, Lane EK, et al. PLoS ONE 8(2), e57151, (2013)
Deletion of iron regulatory protein 1 causes polycythemia and pulmonary hypertension in mice through translational derepression of HIF2α. Ghosh MC, Zhang DL, Jeong SY, et al. Cell Metab. 17(2), 271-81, (2013)
Respiratory complex I deficiency results in low nitric oxide levels, induction of hemoglobin and upregulation of fermentation pathways. Shah JK, Cochrane DW, De Paepe R, et al. Plant Physiol. Biochem. 63, 185-90, (2013)
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