A5545 Sigma

Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, GR-GZ, Guinea Pig Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:50,000-1:80,000
  dot blot: 1:50,000-1:100,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800
shipped in   dry ice
storage temp.   −20°C

Description

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal

Application

BHK-21 cell lysates were analyzed by western blot using HRP-conjugated rabbit anti-guinea pig IgG as the secondary antibody at a 1:10000 dilution in 0.1% tween/PBS. 1

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