Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

Immunogen

Purified rat IgG

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol, and 15 mM sodium azide.

Application

A concentration of 1:10,000 in direct ELISA and 1:5000 in immunoblotting was used to detect salmon thrombin and fibrinogen in the blood of Wistar rats. ¹ The antibody may be used for immunohistochemistry at a concentration of 1:50-1:100 determined by indirect assay using formalin fixed paraffin embedded human tonsil sections and rat Monoclonal Anti-Human IgG as primary antibody. ²

Anti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in ELISA. The product can also be used for immunohistochemistry (at 1:50-1:100 dilutions using formalin-fixed, paraffin-embedded sections) and western blot (1:30,000-1:60,000).

Cell extracts of V. vulnificus strains were analyzed for protein expression by western blot using alkaline phosphatase-conjugated rabbit anti-rat IgG as the secondary antibody at a dilution of 1:1000.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c³. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins. Anti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody binds to all rat immunoglobulins.

The affinity isolated antibody is obtained from anti-rat IgG antiserum by immunospecific purification, which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to rat IgG. Anti-Rat IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde
Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation.