A6481 Sigma

Allene Oxide Synthase from Parthenium argentatum

recombinant, expressed in E. coli, 25,000-40,000 units/mg protein

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Synonym: AOS, Allene Oxide Synthase Parthenium argentatum

  •  Enzyme Commission (EC) Number 4.2.1.92  ( BRENDA | IUBMB )

Properties

Related Categories 4.2.x.x C-O bonds, 4.x.x.x Lyases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
recombinant   expressed in E. coli
packaging   vial of ~300 μg
shipped in   dry ice
storage temp.   −20°C

Description

Biochem/physiol Actions

Potent anti-oxidant enzyme to remove lipid hydroperoxides in biological samples.

Allene oxide synthase converts lipoxygenase derived fatty acid hydroperoxides to unstable allene epoxides. In plants, allene oxide is a precursor of jasmonic acid, which is important for growth regulation 2.

Unit Definition

One unit will cause a change in A234nm of 1.0 per minute at pH 7.0 at 25 °C in a 13S-HPOD((13(S)-hydroperoxy-(9Z,11E)-octadecanoic Acid) reduction assay

Physical form

Supplied as a solution in phosphate buffered sale pH 7.2

Application

Allene Oxide Synthase is a potent anti-oxidant enzyme used to remove lipid hydroperoxides in various biological samples. It may also be used to study stress induced gene expression in plants 1. Product A6481 is from Parthenium argentatumand is supplied as a solution in phosphate buffered saline.

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