Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories	Alphabetical Index, Antibodies, Peroxidase Labeled Antibodies, Primary Antibodies, RAB-RAD, Rabbit Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies More...
antibody form	IgG fraction of antiserum
clone	polyclonal
form	buffered aqueous solution
application(s)	direct ELISA: 1:10,000
shipped in	dry ice
storage temp.	−20°C

Description

General description

IgG, a monomer, is an antibody circulating in blood and can assist in phagocytic destruction of foreign microorganism. It plays an important role in Antibody dependent cell mediated cytotoxicity and is also associated with type II and type III hypersensitivity. Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody can be used in pAb-ELISA. Rabbit anti-IgG antibody coupled with peroxidase (1:2500 dilution in TBS, 0.5% BSA) can be used in western blots. This antibody is also used as detector in indirect ELISA for saxitoxin analysis. Goat anti-rabbit IgG Peroxidase conjugate reacted specifically with all rabbit immunoglobulin. ^2,³

Immunogen

Purified rabbit IgG.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.01% thimerosal

Application

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.¹

Immunohistochemistry was performed on frozen sections (10um) of mouse intestine, liver, and spleen using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Prior to incubation with the secondary, sections were treated with a mixture of MeOH/hydrogen peroxide 30% to block endogenouse peroxidases.