|Related Categories||1-1B, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling,|
|species reactivity||human, rat, pig, bovine|
|application(s)||indirect immunofluorescence: 1:50|
|western blot: 1:500|
|antibody form||ascites fluid|
|form||buffered aqueous solution|
|mol wt||antigen mol wt 80 kDa|
|shipped in||dry ice|
human ... CAPN2(824)|
rat ... Capn2(29154)
m-calpain from bovine skeletal muscle.
Solution in phosphate buffered saline and contains 0.05% sodium azide.
Epitope mapping studies indicate the epitope is between amino acids 502-699 (domain III/IV) of human m-calpain. The antibody reacts specifically with m-calpain. It does not cross-react with μ-calpain, n-calpain, calmodulin or calpastatin. It is not recommended for immunoprecipitation. By immunoblotting, reactivity is observed with human platelets and erythrocytes, bovine platelets, heart and skeletal muscle and with rat myoblasts, kidney, liver and spleen. By immunofluorescence on pig LLC-PK1 cells, diffuse cytoplasmic staining is observed.
Certificate of Analysis
Certificate of Origin
|Personal Protective Equipment||Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)|
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
Specific increase in calcium-activated neutral protease with low calcium sensitivity (m-calpain) in proerythroblastic K562 cell line cells induced to differentiation by phorbol 12-myristate 13-acetate. Nakamura, M., et al. Exp. Cell Res. 200, 513-522, (1992)
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