Monoclonal Anti-m-Calpain (Domain III/IV) antibody produced in mouse
clone 107-82, ascites fluid, buffered aqueous solution
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MDL number MFCD04041214
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Properties
| Related Categories | 1-1B, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, |
| species reactivity | human, rat, pig, bovine |
| application(s) | indirect immunofluorescence: 1:50 |
| western blot: 1:500 | |
| clone | 107-82, monoclonal |
| antibody form | ascites fluid |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen mol wt 80 kDa |
| shipped in | dry ice |
| storage temp. | −20°C |
| Gene Information |
human ... CAPN2(824) rat ... Capn2(29154) |
Description
Immunogen
m-calpain from bovine skeletal muscle.
Physical form
Solution in phosphate buffered saline and contains 0.05% sodium azide.
Specificity
Epitope mapping studies indicate the epitope is between amino acids 502-699 (domain III/IV) of human m-calpain. The antibody reacts specifically with m-calpain. It does not cross-react with μ-calpain, n-calpain, calmodulin or calpastatin. It is not recommended for immunoprecipitation. By immunoblotting, reactivity is observed with human platelets and erythrocytes, bovine platelets, heart and skeletal muscle and with rat myoblasts, kidney, liver and spleen. By immunofluorescence on pig LLC-PK1 cells, diffuse cytoplasmic staining is observed.
Price and Availability
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