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C9017 Sigma

Concanavalin A from Canavalia ensiformis (Jack bean)

Sepharose® conjugate, buffered aqueous suspension

DOWNLOAD MSDS (PDF)

Synonym: ConA

Properties

Related Categories Affinity Chromatography, Bioactive Small Molecules, CI-CY, Carbohydrate Binding Matrices, Cell Biology,
form   buffered aqueous suspension
contains   20% ethanol as preservative
extent of labeling   10-16 mg per mL
matrix   Sepharose 4B
matrix activation   cyanogen bromide
matrix attachment   amino
matrix spacer   1 atom
capacity   ≥25 mg/mL binding capacity (thyroglobulin)
conjugate   Sepharose® conjugate
storage temp.   2-8°C
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Description

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Application

Used for the purification of glycoproteins containing mannose.

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Packaging

25, 100 mL in glass bottle

Physical form

Suspension in 0.1 M acetate buffer, pH 6.0, containing 1 M NaCl, and 1 mM each of CaCl2 , MgCl2, and MnCl2

Legal Information

Sepharose is a registered trademark of GE Healthcare

Price and Availability

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