|Related Categories||Cell Lysis and Protein Extraction, Molecular Biology, Native Protein Sample Preparation, Proteomics, Recombinant Protein Expression and Analysis,|
|shipped in||dry ice|
The kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris, and Schizosaccharomyces pombe yeast strains. The procedure employs spheroplast formation by enzymatic digestion of the yeast cell wall followed by cell protein extraction (with or without detergent), while avoiding protein degradation and interference with protein immunoreactivity and biological activity.
The cell extract is compatible with:
• Reporter gene expression assays (e.g., β-galactosidase, alkaline-phosphatase)
• Immunoassays (Western blots, immunoprecipitation)
• Affinity based purification (e.g. FLAG, glutathione S- transferase (GST) and histidine-tagged fusion proteins)
• DNA-protein interaction assay (e.g., gel-shift)
• Coomassie and silver staining
The kit is suitable for spheroplast formation and protein extraction from 10-40 grams of yeast cell pellet.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
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|Reaction Buffer 120 ml|
|1 M DTT 5 ml|
|Lyticase 25,000 units|
|Extraction buffer 60 ml|
|Triton™ X-100 10 ml|
|Protease inhibitor cocktail 2 ml|
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