D2438 Sigma

Dimethyl sulfoxide

sterile-filtered, Biotechnology Performance Certified, meets EP, USP testing specifications, suitable for hybridoma

DOWNLOAD MSDS (PDF)

Synonym: DMSO, Methyl sulfoxide

  • CAS Number 67-68-5

  • Linear Formula (CH3)2SO

  • Molecular Weight 78.13

  •  Beilstein Registry Number 506008

  •  EC Number 200-664-3

  •  MDL number MFCD00002089

  •  PubChem Substance ID 24893688

  • Popular Documents:  FTNMR (PDF)  

Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Freezing Media and Reagents, Cell Signaling and Neuroscience,
Quality Level   GMP
grade   Biotechnology Performance Certified
vapor density   2.7 (vs air)
vapor pressure   0.42 mmHg ( 20 °C)
sterility   sterile-filtered
assay   (USP XXIV)
autoignition temp.   573 °F
expl. lim.   42 %, 63 °F
refractive index   n20/D 1.479(lit.)
bp   189 °C(lit.)
mp   16-19 °C(lit.)
density   1.10 g/mL(lit.)
suitability   meets EP, USP testing specifications
  suitable for hybridoma

Description

Frequently Asked Questions

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Application

Human and animal cell lines grown in culture are generally stored frozen. Freezing protects the cell line from changes due to genetic drift and minimizes risk of contamination. Liquid nitrogen used in conjunction with a cryoprotective agent such as DMSO is a widely used method for preserving cells. Without the presence of a cryoprotective agent, freezing is lethal to most mammalian cells. Damage is caused by mechanical injury by ice crystals, concentration of electrolytes, pH changes, and denaturation of proteins. These lethal effects are minimized by adding a cryoprotective agent which lowers the freezing point and allows for a slower cooling rate. BPC-tested DMSO also meets the requirements of biotechnology and tissue engineering applications.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Features and Benefits

Ready-to-use sterile filtered product conveniently packaged septum screw-capped amber bottles. Meets USP and EP testing requirements

Price and Availability

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