Enolase from rabbit muscle
ammonium sulfate suspension, ≥15 units/mg protein
DOWNLOAD MSDS (PDF)Synonym:
2-Phospho-
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CAS Number 9014-08-8
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EC Number 232-754-3
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MDL number MFCD00131019
| Properties
| Related Categories | 4.2.x.x C-O bonds, 4.x.x.x Lyases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, |
| form | ammonium sulfate suspension |
| foreign activity | lactic dehydrogenase ≤0.1% |
| pyruvate kinase ≤0.1% | |
| storage temp. | 2-8°C |
Description
Biochem/physiol Actions
Enolase is a metalloenzyme that catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase is essential for both glycolysis and gluconeogenesis.
Unit Definition
One unit will convert 1.0 μmole of 2-phosphoglycerate to phospho(enol)pyruvate per min at pH 7.4 at 25 °C.
Physical form
Crystalline suspension in 2.8 M (NH4)2SO4 containing 0.05 M imidazole and 1 mM MgSO4, pH 7.5
Application
Enolase from rabbit muscle has been used to study its rate-limiting steps and the site of Li+ inhibition.
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