p3XFLAG-CMV™-8 Expression Vector
Shuttle vector for transient expression of secreted N-terminal 3xFLAG fusion proteins
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Properties
| Related Categories | Cloning and Expression, Expression Vectors, For Transient Expression, Mammalian Expression Vectors, Molecular Biology More... |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Other Notes
Browse additional application references in our FLAG® Literature portal.
Application
The p3XFLAG-CMV™-9 Expression Vector (4.8kb) is used to establish expression of secreted N-terminal 3XFLAG® fusion proteins in mammalian cells. The vector encodes three adjacent FLAG® epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. Thisresults in increased detection sensitivity using ANTI-FLAG® M2 antibody. The third epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG® peptide from the purified fusion protein. A p3xFLAG-CMV™-7-BAPcontrol vector is also supplied.
Expression vector and control vector sequences can be found here.
Biochem/physiol Actions
The promoter-regulatory region of the human cytomegalovirus1,2 drives transcription of FLAG®-fusion constructs.
Legal Information
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
Price and Availability
Kit component only
|
Description |
Product # |
Add to Cart |
|---|---|---|
| p3XFLAG-CMV™-8 Expression Vector | ||
| Control Vector, p3xFLAG-CMV™-7-BAP |
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