|Related Categories||Cloning and Expression, Expression Vectors, For Transient Expression, Mammalian Expression Vectors, Molecular Biology More...|
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Browse additional application references in our FLAG® Literature portal.
The p3XFLAG-CMV™-9 Expression Vector (4.8kb) is used to establish expression of secreted N-terminal 3XFLAG® fusion proteins in mammalian cells. The vector encodes three adjacent FLAG® epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. Thisresults in increased detection sensitivity using ANTI-FLAG® M2 antibody. The third epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG® peptide from the purified fusion protein. A p3xFLAG-CMV™-7-BAPcontrol vector is also supplied.
Expression vector and control vector sequences can be found here.
The promoter-regulatory region of the human cytomegalovirus1,2 drives transcription of FLAG®-fusion constructs.
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
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|p3XFLAG-CMV™-8 Expression Vector|
|Control Vector, p3xFLAG-CMV™-7-BAP|
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2. Two-stage selection of sequences from a random phage display library delineates both core residues and permitted structural range within an epitope. Miceli, R.M., et al. J. Immunol. Methods 167, 279-287, (1994)
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