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F4890 Sigma

Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse

clone RG-16, purified immunoglobulin, buffered aqueous solution

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Synonym: Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

Properties

Related Categories Alphabetical Index, Antibodies, Clone Index, FITC Antibodies, Primary Antibodies,
antibody form   purified immunoglobulin
clone   RG-16, monoclonal
form   buffered aqueous solution
does not react with   guinea pig, turkey, rat, goat, canine, feline, bovine, sheep, chicken, horse, human, pig
storage condition   protect from light
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
  indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
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Description

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

General description

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Specificity

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Physical form

Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.1

Price and Availability

Anti-Mouse 2° Antibody Best Sellers

Prod. No.ConjugateSecondary Antibody Description
A4416HRPAnti-Mouse IgG (whole molecule)-Peroxidase
A3562APAnti-Mouse IgG (whole molecule)-Alkaline Phosphatase
F0257FITCAnti-Mouse IgG (whole molecule)-FITC
B7264BiotinAnti-Mouse IgG (whole molecule)-Biotin





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