Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat

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Other Notes

Antibody adsorbed with human IgG.

Immunogen

Purified mouse IgG, Fab fragment

Preparation Note

Adsorbed to reduce background staining with human samples.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Mouse IgG (Fab specific)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a minimum dilution of 1:128. It may be used for imunohistochemistry of formalin-fixed, paraffin-embedded human tonsil slides at a working antibody dilution of 1:150. In an agar diffusion assay the conjugate produces a precipitation arc at a dilution of 1:32 versus a 1:80 dilution of normal mouse serum. The antibody was used at a dilution range of 1:50 to 1:300 for immunofluorescence in various studies.^1,2,3

Goat polyclonal anti-Mouse IgG (Fab specific)−FITC antibody may be used to detect and quantitate the level of IgG in mouse serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use mouse IgG as the primary antibody.

General description

Goat polyclonal anti-Mouse IgG (Fab specific)−FITC antibody reacts with all mouse IgGs. It shows no reactivity with mouse IgG, Fc fragment, or human IgG. Identity and purity of the antibody is established by immunoelectrophoresis, prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (Fab specific)-FITC antibody is specific for Fab fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG, Fc fragment, or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. The antibody is adsorbed with human IgG to ensure minimal cross reactivity.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.