Fructose-6-phosphate Kinase, Pyrophosphate-dependent from Propionibacterium freudenreichii (shermanii)
lyophilized powder, 4.0-8.0 units/mg protein
DOWNLOAD MSDS (PDF)Synonym:
6-
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CAS Number 55326-40-4
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MDL number MFCD00131108
| Properties
| Related Categories | 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Carbohydrate Metabolism, Enzyme Class Index, |
| form | lyophilized powder |
| composition | Protein, 5.0-25.0% biuret |
| storage temp. | −20°C |
Description
Other Notes
These two pyrophosphate-dependent fructose-6-phosphate kinases differ in their response to the physiological activator fructose 2,6-diphosphate [Fru (2,6)-P2]. The enzyme from Propionibacterium freudenreichii (F8381) reportedly is not affected by Fru (2,6)-P2.3 The enzyme from potato tubers (F2258) is stimulated by Fru (2,6)-P2 (half-maximal activation at 5.5 nM Fru (2,6)-P2 for potato tuber enzyme).4
Unit Definition
One unit will convert 1.0 μmole of pyrophosphate and fructose 6-phosphate to fructose 1,6-diphosphate and inorganic phosphate per min at pH 7.4 at 30 °C.
Physical form
Contains imidazole salts and stabilizer
Application
Can be used for the determination of pyrophosphate by the method of O′Brien, W., Anal. Biochem., FB76, 423 (1976).
Biochem/physiol Actions
Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle1,2.
Price and Availability
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