|Related Categories||2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Carbohydrate Metabolism, Cell Biology,|
|composition||Protein, 5.0-25.0% biuret|
These two pyrophosphate-dependent fructose-6-phosphate kinases differ in their response to the physiological activator fructose 2,6-diphosphate [Fru (2,6)-P2]. The enzyme from Propionibacterium freudenreichii (F8381) reportedly is not affected by Fru (2,6)-P2.3 The enzyme from potato tubers (F2258) is stimulated by Fru (2,6)-P2 (half-maximal activation at 5.5 nM Fru (2,6)-P2 for potato tuber enzyme).4
One unit will convert 1.0 μmole of pyrophosphate and fructose 6-phosphate to fructose 1,6-diphosphate and inorganic phosphate per min at pH 7.4 at 30 °C.
Contains imidazole salts and stabilizer
Can be used for the determination of pyrophosphate by the method of O′Brien, W., Anal. Biochem., FB76, 423 (1976).
Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle1,2.
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≥98%, amorphous powder
Type VII, lyophilized powder, ≥50 units/mg protein
lyophilized powder, ≥2 units/mg protein (Lowry)
|Precautionary statements||P280-P305 + P351 + P338-P310|
|Personal Protective Equipment||dust mask type N95 (US), Eyeshields, Faceshields, Gloves|
|Hazard Codes (Europe)||Xi|
|Risk Statements (Europe)||36/38|
|Safety Statements (Europe)||26|
1. OBJECTIVE To standardize a procedure for the enzymatic assay of Fructose -6- Phosphate Kinase (Pyrophosphate Dependent) from Propionibacterium Freudenreichii (Shermanii).
Keywords: Atomic layer deposition, Extinction coefficient
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
3. A kinetic study of pyrophosphate: fructose-6-phosphate phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate. VanSchaftingen, E., et al. Eur. J. Biochem. 129, 191, (1982)
4. D-Fructose 2,6-bisphosphate: a naturally occurring activator for inorganic pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase in plants. Sabularse, D.C. and Anderson, R.L. Biochem. Biophys. Res. Commun. 103, 848, (1981)
Physiological relevance of fructose 2,6-bisphosphate in the regulation of spinach leaf pyrophosphate:fructose 6-phosphate 1-phosphotransferase. Theodorou ME and Kruger NJ Planta 213(1), 147-57, (2001)
Presence of prokaryotic and eukaryotic species in all subgroups of the PP(i)-dependent group II phosphofructokinase protein family. Müller M, Lee JA, Gordon P, et al. J. Bacteriol. 183(22), 6714-6, (2001)
Different physiological roles of ATP- and PP(i)-dependent phosphofructokinase isoenzymes in the methylotrophic actinomycete Amycolatopsis methanolica. Alves AM, Euverink GJ, Santos H, et al. J. Bacteriol. 183(24), 7231-40, (2001)
Tobacco transformants with strongly decreased expression of pyrophosphate:fructose-6-phosphate expression in the base of their young growing leaves contain much higher levels of fructose-2,6-bisphosphate but no major changes in fluxes. Nielsen TH and Stitt M Planta 214(1), 106-16, (2001)
Fluorescence study of ligand binding to potato tuber pyrophosphate-dependent phosphofructokinase: evidence for competitive binding between fructose-1,6-bisphosphate and fructose-2,6-bisphosphate. Podestá FE and Plaxton WC Arch. Biochem. Biophys. 414(1), 101-7, (2003)
In silico exploration of the fructose-6-phosphate phosphorylation step in glycolysis: genomic evidence of the coexistence of an atypical ATP-dependent along with a PPi-dependent phosphofructokinase in Propionibacterium freudenreichii subsp. shermanii. Meurice G, Deborde C, Jacob D, et al. In Silico Biol. (Gedrukt.) 4(4), 517-28, (2004)
Identification and cloning of the gene encoding pyrophosphate-dependent 6-phosphofructokinase of Methylomonas methanica. Reshetnikov AS, Mustakhimov II, Khmelenina VN, et al. Dokl. Biochem. Biophys. 405, 468-70, (2005)
Identification of a 20-bp regulatory element of the Arabidopsis pyrophosphate:fructose-6-phosphate 1-phosphotransferase alpha2 gene that is essential for expression. Lim HM, Cho JI, Lee S, et al. Plant Cell Rep. 26(5), 683-92, (2007)
Down-regulation of pyrophosphate: fructose 6-phosphate 1-phosphotransferase (PFP) activity in sugarcane enhances sucrose accumulation in immature internodes. Groenewald JH and Botha FC Transgenic Res. 17(1), 85-92, (2008)
A strategy for designing multi-taxa specific reference gene systems. example of application--ppi phosphofructokinase (ppi-PPF) used for the detection and quantification of three taxa: maize (Zea mays), cotton (Gossypium hirsutum) and rice (Oryza sativa). Chaouachi M, Giancola S, Romaniuk M, et al. J. Agric. Food Chem. 55(20), 8003-10, (2007)
Transposable elements in Coffea (Gentianales: Rubiacea) transcripts and their role in the origin of protein diversity in flowering plants. Lopes FR, Carazzolle MF, Pereira GA, et al. Mol. Genet. Genomics 279(4), 385-401, (2008)
Examination of intrinsic sulfonamide resistance in Bacillus anthracis: a novel assay for dihydropteroate synthase. Valderas MW, Andi B, Barrow WW, et al. Biochim. Biophys. Acta 1780(5), 848-53, (2008)
Downregulation of pyrophosphate: D-fructose-6-phosphate 1-phosphotransferase activity in sugarcane culms enhances sucrose accumulation due to elevated hexose-phosphate levels. van der Merwe MJ, Groenewald JH, Stitt M, et al. Planta 231(3), 595-608, (2010)
Identification and characterization of a null-activity mutant containing a cryptic pre-mRNA splice site for cytosolic fructose-1,6-bisphosphatase in Flaveria linearis. Slater, S.M., et al. Plant Mol. Biol. 74, 519-36, (2010)
Characterization of the recombinant pyrophosphate-dependent 6-phosphofructokinases from Methylomicrobium alcaliphilum 20Z and Methylococcus capsulatus Bath. Khmelenina VN, Rozova ON, and Trotsenko YA Meth. Enzymol. 495, 1-14, (2011)
Pyrophosphate-Dependent Fructose-6-Phosphate 1-Phosphotransferase Induction and Attenuation of Hsp Gene Expression during Endosperm Modification in Quality Protein Maize. Guo X, Ronhovde K, Yuan L, Yao B, et al. Plant Physiol. 158, 917-929, (2012)
Pyrophosphate:D-fructose 6-phosphate 1-phosphotransferase. A new enzyme with the glycolytic function of 6-phosphofructokinase. Reeves RE, South DJ, Blytt HJ, et al. J. Biol. Chem. 249(24), 7737-41, (1974)
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