|Related Categories||2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Carbohydrate Metabolism, Enzyme Class Index,|
|composition||Protein, 5.0-25.0% biuret|
These two pyrophosphate-dependent fructose-6-phosphate kinases differ in their response to the physiological activator fructose 2,6-diphosphate [Fru (2,6)-P2]. The enzyme from Propionibacterium freudenreichii (F8381) reportedly is not affected by Fru (2,6)-P2.3 The enzyme from potato tubers (F2258) is stimulated by Fru (2,6)-P2 (half-maximal activation at 5.5 nM Fru (2,6)-P2 for potato tuber enzyme).4
One unit will convert 1.0 μmole of pyrophosphate and fructose 6-phosphate to fructose 1,6-diphosphate and inorganic phosphate per min at pH 7.4 at 30 °C.
Contains imidazole salts and stabilizer
Can be used for the determination of pyrophosphate by the method of O′Brien, W., Anal. Biochem., FB76, 423 (1976).
Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle1,2.
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≥98%, amorphous powder
Type VII, lyophilized powder, ≥50 units/mg protein
lyophilized powder, ≥2 units/mg protein (Lowry)
|Precautionary statements||P280-P305 + P351 + P338-P310|
|Personal Protective Equipment||dust mask type N95 (US), Eyeshields, Faceshields, Gloves|
|Hazard Codes (Europe)||Xi|
|Risk Statements (Europe)||36/38|
|Safety Statements (Europe)||26|
1. OBJECTIVE To standardize a procedure for the enzymatic assay of Fructose -6- Phosphate Kinase (Pyrophosphate Dependent) from Propionibacterium Freudenreichii (Shermanii).
Keywords: Atomic layer deposition, Extinction coefficient
3. A kinetic study of pyrophosphate: fructose-6-phosphate phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate. VanSchaftingen, E., et al. Eur. J. Biochem. 129, 191, (1982)
4. D-Fructose 2,6-bisphosphate: a naturally occurring activator for inorganic pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase in plants. Sabularse, D.C. and Anderson, R.L. Biochem. Biophys. Res. Commun. 103, 848, (1981)
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