Factor X Activated (Xa) from bovine plasma aqueous glycerol solution

Properties

Related Categories	3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Blood, Cell Biology, Cell Signaling and Neuroscience, Clotting Factors, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, Factor Xa, Factors, Fusion Tag and Protease Removal, Immune Cell Signaling and Blood, Plasma & Blood Proteins, Plasma, Blood, and Related Proteins and Reagents, Proteases & Protein Sequencing, Proteases for Recombinant Protein Fusion Tag Cleavage, Proteins and Derivatives, Proteolytic Enzymes and Substrates, Proteomics, Purification and Detection, Recombinant Protein Expression and Analysis, Selective Proteolytic Enzymes More...
form	aqueous glycerol solution
shipped in	wet ice
storage temp.	−20°C
Gene Information	cow ... F10(280787)

Description

Unit Definition

One unit of activated Factor X will liberate 1.0 μmole of p-nitroanilide from N-benzoyl-L-isoleucyl-L-glutamyl-L-glycyl-L-arginine-p-nitroaniline per minute at pH 8.3 at 37 °C.

Application

Fusion proteins are commonly expressed with a factor Xa cleavable Ile-Glu (or Asp)-Gly-Arg-↓-X sequence. Typically 1 mg of fusion protein can be incubated with 10 μg of factor Xa for 2.5 hours at 37 °C.

Biochem/physiol Actions

Factor Xa catalyzes the hydrolysis of the Arg-Thr and then Arg-Ile bonds in prothrombin to yield active thrombin.
The fairly strict recognition sequence is Ile-Glu (or Asp)-Gly-Arg-↓-X.
It may sometimes cleave at other basic residues, depending on the conformation of the target protein. Factor Xa will not cleave if a proline residue follows the arginine of the recognition sequence.
pH Optimum: 7.6-8.0
Temperature Optimum: 37 °C

Physical properties

Factor Xa is a serine endoproteinase and a member of the S1 peptidase family. Factor Xa plays a critical role in the coagulation cascade by catalyzing the proteolytic conversion of prothrombin to active thrombin. Factor Xa′s prothrombin conversion activity is greatly enhanced in vivo when complexed with factor V, calcium ions and phospholipids on the activated platelet surface.
The zymogen form, Factor X, is activated in vivo by two different pathways. The intrinsic pathway utilizes a catalytic complex composed of factor IXa, factor VIII, phospholipids and calcium ions. The extrinsic pathway utilizes a complex of factor VII and tissue factor. The factor X zymogen is a 55 KDa glycoprotein with a light and heavy chain joined by a single disulfide.

Price and Availability

Related Papers

Hemostatic properties of a venomic protein in rat organ trauma. Roscoe L. Warner et al Exp. Mol. Pathol. 87, 204-11, (2009)

Documents

Certificate of Analysis

Certificate of Origin

Enzyme Explorer

Proteinase-Activated Receptors (172 KB )

Safety Information

WGK Germany

1

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Gas chromatography

Papers

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Hemostatic properties of a venomic protein in rat organ trauma. Roscoe L. Warner et al Exp. Mol. Pathol. 87, 204-11, (2009)

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Enzymatic Cleavage of Fusion Proteins with Factor Xa. La Vallie, E.R., and McCoy, J.M., Ausubel, F.M., ed. Curr. Protoc. Mol. Biol., (1994) 2, 16.4.6-16.4.7