|Related Categories||1.11.x.x Peroxide as receptor, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Biology,|
|mol wt||mol wt 84.5 kDa|
|composition||Protein, 10-30% modified Warburg-Christian|
|shipped in||dry ice|
Protects cells against oxidative damage by catalyzing the reduction of hydrogen peroxide in the presence of reducing agent glutathione.3 In cellular membranes it may induce lipid peroxidation through the reduction of hydrogen peroxide or polyunsaturated fatty acid hydroperoxides. 4
Glutathione peroxidase is an enzyme which reduced lipid hydroperoxides into their corresponding alcohols. It also reduces free hydrogen peroxide in to water. In vivo it is responsible for protecting hemoglobin from oxidative breakdown1,2.
Note: At the reported pH optimum of 8.8, we have found the activity to be approx. 10 times that at pH 7.0. However, to remain consistent with literature and avoid complications arising from non-enzymatic oxidation of GSH, our unit is defined at pH 7.0.
One unit will catalyze the oxidation by H2O2 of 1.0 μmole of reduced glutathione to oxidized glutathione per min at pH 7.0 at 25 °C.
Lyophilized powder containing 25% sucrose and 2.5% dithiothreitol with sodium phosphate buffer salts
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Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
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Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is ...
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3. The role of selenium peroxidases in the protection against oxidative damage Ursini F. & Bindoli A. Chem. Phys. Lett. 44, 255-276, (1987)
Wendel, A., Jacoby, W.B., ed. Enzymatic Basis of Detoxification New York , (1980), 333
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|CGP1||Glutathione Peroxidase Cellular Activity Assay Kit|
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