Glutathione Peroxidase from bovine erythrocytes lyophilized powder, ≥300 units/mg protein

Properties

Related Categories	1.11.x.x Peroxide as receptor, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience, Cell Stress, Diagnostic and Analytical Enzymes, Enzyme Class Index, Enzymes, Enzymes, Inhibitors, and Substrates, General Metabolic Enzymes, General Metabolic Enzymes A-H, Metabolomics, Nitric Oxide and Cell Stress, Oxidative Stress Enzymes More...
form	lyophilized powder
mol wt	mol wt 84.5 kDa
composition	Protein, 10-30% modified Warburg-Christian
shipped in	dry ice
storage temp.	−20°C

Description

Biochem/physiol Actions

Protects cells against oxidative damage by catalyzing the reduction of hydrogen peroxide in the presence of reducing agent glutathione.³ In cellular membranes it may induce lipid peroxidation through the reduction of hydrogen peroxide or polyunsaturated fatty acid hydroperoxides. ⁴

Glutathione peroxidase is an enzyme which reduced lipid hydroperoxides into their corresponding alcohols. It also reduces free hydrogen peroxide in to water. In vivo it is responsible for protecting hemoglobin from oxidative breakdown^1,².

Other Notes

Note: At the reported pH optimum of 8.8, we have found the activity to be approx. 10 times that at pH 7.0. However, to remain consistent with literature and avoid complications arising from non-enzymatic oxidation of GSH, our unit is defined at pH 7.0.

Unit Definition

One unit will catalyze the oxidation by H₂O₂ of 1.0 μmole of reduced glutathione to oxidized glutathione per min at pH 7.0 at 25 °C.

Physical form

Lyophilized powder containing 25% sucrose and 2.5% dithiothreitol with sodium phosphate buffer salts

Price and Availability

Related Papers

1. The refined structure of the selenoenzyme glutathione peroxidase at 0.2-nm resolution O. Epp et al. Eur. J. Biochem. 133, 51-69, (1983)

Documents

Certificate of Analysis

Certificate of Origin

Celltransmissions 19(1) (144 KB )

Enzyme Explorer

Safety Information

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany	3

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Cellular Derived Blood Proteins and Enzymes

Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
BioFiles 2006, 1.5, 4.
Keywords: Aerobic, Alzheimer Disease, Bacterial conjugations, Cancer, Metabolism, Oxidations, Parkinson Disease, Reductions, Respiration

Mitochondrial Stress and ROS

Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is ...
BioFiles 2011, 6.4, 22.
Keywords: Anti-inflammatory agents, Apoptosis, Cancer, Cell signaling, Cellular processes, Gene expression, Indicators, Metabolism, Oxidations, Respiratory