L -Glutamine–Penicillin–Streptomycin solution
L -glutamine 200 mM, penicillin 10,000 units, streptomycin 10 mg/mL, sterile-filtered, BioReagent, suitable for cell culture
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MDL number MFCD00283636
Properties
| Related Categories | A - K, Antibacterial, Antibiotic Solutions, Antibiotics, Antibiotics A to Z, |
| description | stabilized |
| sterility | sterile-filtered |
| product line | BioReagent |
| composition |
|
| penicillin, 10,000 units | |
| streptomycin, 10 mg/mL | |
| impurities | endotoxin, tested |
Description
Application
Use to reduce or eliminate bacterial contamination in cell, especially primary cell, cultures. Recommended for use at 10 ml/L.
Biochem/physiol Actions
Mode of Action: Glutamine is essential as an energy source for most mammalian cells in culture because of its amino acid carbon skeleton. Penicillin acts by inhibiting bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome and causes miscoding.
Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.
Other Notes
Solubilized in a proprietary buffer.
Protocols & Applications
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with 200 mM L-glutamine, 10,000 U penicillin and 10 mg steptomycin/mL in 0.9% NaCl, sterile-filtered, BioReagent, suitable for cell culture
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with 10,000 units penicillin and 10 mg streptomycin per mL in 0.9% NaCl, sterile-filtered, BioReagent, suitable for cell culture
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