G7402 Sigma

Anti-Rabbit IgG (whole molecule)–Gold antibody produced in goat

affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)

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Properties

Related Categories Alphabetical Index, Antibodies, Gold, Primary Antibodies, RAB-RAD,
antibody form   affinity isolated antibody
clone   polyclonal
form   aqueous glycerol suspension
availability   not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)
application(s)   dot blot: suitable
  western blot: suitable
particle size   10 nm (colloidal gold)
storage temp.   2-8°C

Description

Immunogen

IgG purified from normal rabbit serum

Rabbit IgG purified from normal rabbit serum.

Physical form

Colloidal suspension in 0.02 M Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide.

Application

Anti-Rabbit IgG (whole molecule)-Gold antibody is suitable for use in immunoelectron microscopy1 and western blot2.

Anti-rabbit IgG (whole molecule)-gold antibody (diluted 1: 15) can be used in immunoelectron microscopy. It can also be used in western blotting.

General description

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding3. Anti-rabbit IgG (whole molecule)-gold antibody can be used as secondary antibody (diluted 1:50 in 0.01 M PBS containing 1% BSA and 5% NGS) in EM immunolabeling of GABA and glutamate by postembedding procedure4. It can also be used in a modified dot blot assay to determine binding activity. Goat anti-rabbit IgG (whole molecule)-gold antibody reacts specifically with all goat immunoglobulins.

Immunoglobulins (Igs) are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs can be useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Gold antibody is specific for rabbit IgG.

Price and Availability

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affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)

affinity isolated antibody, aqueous glycerol suspension, 5 nm (colloidal gold)

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