Hyaluronidase from Streptomyces hyalurolyticus lyophilized powder

Properties

Related Categories	4.2.x.x C-O bonds, 4.x.x.x Lyases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis, Carbohydrate hydrolysis & PTM analysis, Carbohydrate-active Enzymes, Complex Carbohydrate Analysis, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, Glycobiology, Glycosaminoglycan (GAG) Degrading Enzymes, Hyaluronidase (Hyaluronate Lyase), Hyaluronidase and Hyaluronic Acxid, Post-Translational Modification, Proteomics More...
form	lyophilized powder
packaging	ampule of 300 × units
storage temp.	−20°C

Description

Frequently Asked Questions

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Biochem/physiol Actions

Hyaluronate lyase cleaves hyaluronic acid at the β-D-GalNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.¹

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

One unit will cause a change in A₆₀₀ of 0.330 per minute at pH 5.7 at 37°C in a 2.0 ml reaction mixture (45 minute assay).

Price and Availability

Related Papers

1. Novel hyaluronidase from streptomyces. Ohya, T., and Kaneko, Y. Biochim. Biophys. Acta 198, 607, (1970)

Documents

Certificate of Analysis

Certificate of Origin

Ask A Scientist

Carbohydrate Analysis bf8 (1 MB )

Enzyme Explorer

Safety Information

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany	3

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units. The disaccharide units that are the basis of all glycosaminoglycans (except keratan) are a combination...
Vicki Caligur
BioFiles 2008, 3.10, 4.
Keywords: Adhesion, Aminations, Angiogenesis, Anticoagulants, Apoptosis, Atomic absorption spectroscopy, Cancer, Cell biology, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatography, Coagulation, Degradations, Enzyme-linked immunosorbent assay, Epimerizations, Gene expression, Genetic, Genomics, Growth factors, High performance liquid chromatography, Inflammation, Ion-exchange chromatography, Ligands, Mass spectrometry, Microarray Analysis, Morphogenesis, Nuclear magnetic resonance spectroscopy, Phosphorylations, Polymerase chain reaction, Polymerization reactions, Proteomics, Reductive aminations, Spectroscopy, Sulfations, Transcription, transformation