|Related Categories||A - M, All Liquid Media/Broths, Escherichia coli & Coliforms, HIS Select Supporting Products and Reagents, HIS-Select,|
|grade||for molecular biology|
|description||recommended for cultivation of recombinant E. coli.|
|pH range||6.8 - 7.2 (2.8% solution)|
|storage temp.||room temp|
|suitability||nonselective for Escherichia coli and Coliforms|
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20 g/L Tryptone
5 g/L Yeast Extract
2.4 g/L MgSO4
0.186 g/L KCl
Hannahan’s Broth (SOB) powder provides:
• Standard formulation
• An inexpensive alternative to liquid
Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation
From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Sambrook, J., et al. Molecular Cloning: A Laboratory Manual 2nd ed., Plainview, NY , (1989), 1.76-1.81, A.2
Difco Manual 11th ed., Sparks, MD , (1998), 444
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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