Synonym: Heparin Lyase III, Heparitinase I, Heparitinase from Flavobacterium heparinum
|Related Categories||4.2.x.x C-O bonds, 4.x.x.x Lyases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,|
|mol wt||mol wt 70.8 kDa|
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Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.
One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.
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Certificate of Analysis
Certificate of Origin
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units. The disaccharide units that are the basis of all glycosaminoglycans (except keratan) are a combination...
BioFiles 2008, 3.10, 4.
Keywords: Adhesion, Aminations, Angiogenesis, Anticoagulants, Apoptosis, Atomic absorption spectroscopy, Cancer, Cell biology, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatography, Coagulation, Degradations, Enzyme-linked immunosorbent assay, Epimerizations, Gene expression, Genetic, Genomics, Growth factors, High performance liquid chromatography, Inflammation, Ion-exchange chromatography, Ligands, Mass spectrometry, Microarray Analysis, Morphogenesis, Nuclear magnetic resonance spectroscopy, Phosphorylations, Polymerase chain reaction, Polymerization reactions, Proteomics, Reductive aminations, Spectroscopy, Sulfations, Transcription, transformation
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Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
McLean, M.W. Proc. 9th Int. Symp. Glycoconjugates, B-41, (1987)
PDGF-A interactions with fibronectin reveal a critical role for heparan sulfate in directed cell migration during Xenopus gastrulation. Smith EM, Mitsi M, Nugent MA, et al. Proc. Natl. Acad. Sci. U. S. A. 106(51), 21683-8, (2009)
High glucose-mediated loss of cell surface heparan sulfate proteoglycan impairs the endothelial shear stress response. Brower JB, Targovnik JH, Caplan MR, et al. Cytoskeleton (Hoboken.) 67(3), 135-41, (2010)
Intraarticular injection of heparin-binding insulin-like growth factor 1 sustains delivery of insulin-like growth factor 1 to cartilage through binding to chondroitin sulfate. Miller RE, Grodzinsky AJ, Cummings K, et al. Arthritis Rheum. 62(12), 3686-94, (2010)
Shear stress modulation of smooth muscle cell marker genes in 2-D and 3-D depends on mechanotransduction by heparan sulfate proteoglycans and ERK1/2. Shi ZD, Abraham G, and Tarbell JM PLoS ONE 5(8), e12196, (2010)
Heparan sulfate acts as a bone morphogenetic protein coreceptor by facilitating ligand-induced receptor hetero-oligomerization. Kuo WJ, Digman MA, and Lander AD Mol. Biol. Cell 21(22), 4028-41, (2010)
Characterization of currently marketed heparin products: reversed-phase ion-pairing liquid chromatography mass spectrometry of heparin digests. Brustkern AM, Buhse LF, Nasr M, et al. Anal. Chem. 82(23), 9865-70, (2010)
Degradation of the endothelial glycocalyx is associated with chylomicron leakage in mouse cremaster muscle microcirculation. Constantinescu A, Spaan JA, Arkenbout EK, et al. Thromb. Haemost. 105(5), 790-801, (2011)
Stiffness and heterogeneity of the pulmonary endothelial glycocalyx measured by atomic force microscopy. O'Callaghan R, Job KM, Dull RO, et al. Am. J. Physiol. Lung Cell. Mol. Physiol. 301(3), L353-60, (2011)
Heparan sulfate proteoglycan mediates shear stress-induced endothelial gene expression in mouse embryonic stem cell-derived endothelial cells. Nikmanesh M, Shi ZD, and Tarbell JM Biotechnol. Bioeng. 109(2), 583-94, (2012)
Syndecans reside in sphingomyelin-enriched low-density fractions of the plasma membrane isolated from a parathyroid cell line. Podyma-Inoue KA, Hara-Yokoyama M, Shinomura T, et al. PLoS ONE 7(3), e32351, (2012)
Unchanged distribution density of anionic sites on the glomerular wall in rats with streptozotocin-induced diabetic nephropathy. Shimoi A, Hatakeyama H, Koizumi H, et al. Toxicol. Pathol. 40(5), 789-96, (2012)
Use of reflectance interference contrast microscopy to characterize the endothelial glycocalyx stiffness. Job KM, Dull RO, and Hlady V Am. J. Physiol. Lung Cell. Mol. Physiol. 302(12), L1242-9, (2012)
Degradation of heparan sulfate proteoglycans enhances oxidized-LDL-mediated autophagy and apoptosis in human endothelial cells. Ding Z, Wang X, Khaidakov M, et al. Biochem. Biophys. Res. Commun. 426(1), 106-11, (2012)
Overall sulfation of heparan sulfate from pancreatic islet β-TC3 cells increases maximal fibril formation but does not determine binding to the amyloidogenic peptide islet amyloid polypeptide. Hull RL, Peters MJ, Perigo SP, et al. J. Biol. Chem. 287(44), 37154-64, (2012)
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