|Related Categories||Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Cell Lysis and Protein Extraction Reagents, Core Bioreagents,|
|transition temp||cloud point 63-69 °C|
Used in denaturing (urea) IEF and two-dimensional electrophoresis.1 Helps facilitate extrusion of the IEF capillary gels from the tubes.2 Used extensively with immobilized pH gradient (IPG) gels.3 Concentrations between 2-4% (v/v) are typically used in an IEF gel.
May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.
Nonionic, non-denaturing detergent
25, 50, 100 mL in glass bottle
Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.
IGEPAL is a registered trademark of Rhodia Operations
Certificate of Analysis
|Precautionary statements||P280-P305 + P351 + P338|
|Personal Protective Equipment||Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter|
|Hazard Codes (Europe)||Xi|
|Risk Statements (Europe)||41|
|Safety Statements (Europe)||26-39|
Physiological properties of enkephalin-containing neurons in the spinal dorsal horn visualized by expression of green fluorescent protein in BAC transgenic mice. Fukushima T BMC Neurosci. 12, 36, (2011)
Application of sequential extraction procedures and glycoprotein blotting for the characterization of the 2-D polypeptide patterns of barley seed proteins. Weiss, W., et al. Electrophoresis 13, 770-773, (1992)
A universal method for two-dimensional polyacrylamide gel electrophoresis of membrane proteins using isoelectric focusing on immobilized pH gradients in the first dimension. Schupbach, J., et al. Anal. Biochem. 196, 337-343, (1991)
Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension of barley seed proteins: discrimination of cultivars with different malting grades. Gorg, A., et al. Electrophoresis 13, 192-203, (1992)
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