|Related Categories||Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neurotransmission, Antibodies to Ion Channels,|
|species reactivity||human (denatured), rat (denatured), chicken|
|application(s)||immunohistochemistry (frozen sections): 1:3,000|
|clone||mAb 306, monoclonal|
|antibody form||purified immunoglobulin|
|form||buffered aqueous solution|
|shipped in||dry ice|
human ... CHRNA7(1139)|
rat ... Chrna7(25302)
native and denatured α7 subunit (amino acids 380-400, binds α-bungarotoxin) of the nicotinic acetylcholine receptor from chicken and the denatured α7 subunit from rat.
Nicotinic acetylcholine receptors (nAChRs) function as ligand-gated channels that modulate endoplasmic reticulum stress and neurotransmitter release. These receptors also control synaptic signaling in ganglia and muscles. The α7 form of nAChR is expressed in mitochondria and regulates Ca2+ transport and the release of cytochrome c. When expressed in non-excitable cells, α7 nAChR mediates cell growth and survival 2,3
Monoclonal anti-nicotinic acetylcholine receptor ((α7) subunit) antibody is specific for native and denatured α7 subunits in chick, as well as for the denatured α7 subunits in rats and humans. The antibody does not bind well to the native form of the mammalian α7 subunit. The product can be used in chick, rat and human tissues.
Solution in 20 mM Na3PO4, pH 7.2, containing 150 mM NaCl and 0.05% sodium azide.
Monoclonal anti-nicotinic acetylcholine receptor ((α7) subunit) antibody is suitable for use in immunocytochemistry (at 1:3000-1:50 dilutions using paraformaldehyde fixed, cultured rat chromaffin cells) 1, and immunohistochemistry (at 1:3,000 dilutions using frozen sections).
Sold with permission of the Salk Institute
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|Personal Protective Equipment||Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)|
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
2. Mitochondria express α7 nicotinic acetylcholine receptors to regulate Ca2+ accumulation and cytochrome c release: study on isolated mitochondria. Gergalova, G., et al. PLoS ONE 7(2), e31361, (2012)
Britto, L., et al. J. Comp. Neurol. 350, 325, (1994)
Lindstrom, J. Neurotransmissions Natick, MA 12, (1996)
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