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MTOX1018 Sigma

BSEP Knockout HepaRG Cells

1 vial

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Properties

Related Categories ADME/Tox Products, Cell Biology, Cell Line Models, Liver Drug Transporter/Toxicity Models
biological source   Liver from human female (Source Disease: Hepatocarcinoma and Hepatitis C)
storage temp.   −196°C
Gene Information   human ... ABCB11(1351619)

Description

Application

See technical bulletin for detailed protocols

Features and Benefits

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, cholestasis, and hepatotoxicity.

• Sigma′s HepaRG BSEP Knockout (KO) allows investigations of drug-transporter interactions and bile acid biosynthesis, accumulation (Cholestasis), and transport involving BSEP in the liver.

Zinc finger nucleases (ZFN) mediated Knock-out of ABCB11 (BSEP) gene.

• The frame-shift mutation of ABCB11 gene was confirmed by fragment length analysis and DNA sequencing.
• Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.

General description

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG BSEP knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Legal Information

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.

HepaRG is a trademark of BioPredic International company

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Price and Availability


Hepatocyte

All labs need water
Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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